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pFA6a-mAID-mNeon-HIS3MX6

Plasmid

#247601

Purpose

Yeast gene tagging with mAID

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mAID

Tags

mNeonGreen

Expression

Yeast

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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EML6428_ pRL662-eGFP

Plasmid

#234350

Purpose

Expressed eGFP(S65T) in agrobacteria for tracing in planta

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

SpeR

Expression

Bacterial

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1P_EGFP

Plasmid

#232171

Purpose

EGFP expression under the control of the human Synapsin-1 promoter

Depositor

Michael Wehr

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-M1-BRG1-3xFLAG

Plasmid

#109188

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Insert

BRG1 (SMARCA4 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4 [luc2P/IL-2/Hygro] Vector

Plasmid

#236930

Purpose

Express IL-2 in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

IL-2 (IL2 Human)

Use

Luciferase

Mutation

None

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET-28a KGA-WT

Plasmid

#227846

Purpose

To express in E. coli KGA-WT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

KGA isoform (GLS Human)

Tags

HIS

Expression

Bacterial

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a GAC-WT

Plasmid

#227845

Purpose

To express in E. coli GAC-WT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

GAC isoform (GLS Human)

Tags

HIS

Expression

Bacterial

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT28

Plasmid

#223400

Purpose

T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-rtTA

Plasmid

#233202

Purpose

Retroviral expression of rtTA for dox-inducible activation from a TRE promoter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

rtTA

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-zH2B-GFP

Plasmid

#226561

Purpose

Express H2B-GFP in zebrafish.

Depositor

Yong Zhang

Insert

h2b (h2bc1 Zebrafish)

Use

Synthetic Biology; Zebrafish expression

Tags

EGFP

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)

Plasmid

#233542

Purpose

A CMV promoter driving expression of a murine dominant negative myc tagged TCF4.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF4 (TCF4 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

lacks aa 1-56

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1316 - pAAV-AiE2010m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230526

Purpose

AiE2010m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAM102

Plasmid

#226694

Purpose

6xHis-Sumo-Ub-Ub-Ub-Ub

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

UB (UBI4 Budding Yeast)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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Ubc-c005

Plasmid

#216876

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.28.3z

Plasmid

#200678

Purpose

Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGreenFire1_E2F1RE

Plasmid

#112248

Purpose

expression of GFP and Luciferase under the control of E2F1 responsive element

Depositor

Simone Di Giovanni

Insert

E2F1RE (responsive element)

Use

Lentiviral

Tags

copGFP and luciferase

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH2-R172K-FLAG

Plasmid

#66807

Purpose

Tet-inducible expression of mutant IDH2 in mammalian cells

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH2 (IDH2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

R172K

Promoter

CMV

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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