164,577 results

1. FLAG-mBAF45c IRES GFP

   Plasmid

   #17882

   Depositor

   Jerry Crabtree

   Article

   Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

   Insert

   BAF45c (Dpf3 Mouse)
   

   Use

   Tags

   FLAG and IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pCDH-3xFLAG-TERT

   Plasmid

   #51631

   Purpose

   Mammalian expression of 3xFLAG tagged TERT, N-terminal.

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-mBAF45b IRES GFP

   Plasmid

   #17881

   Depositor

   Jerry Crabtree

   Article

   Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

   Insert

   BAF45b (Dpf1 Mouse)
   

   Use

   Tags

   FLAG and IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

4. CROP_C9_Puro_PDGFRB

   Plasmid

   #183314

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PDGFRB
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1682

   Plasmid

   #142825

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SIM1 (SIM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Th-iCre

   Plasmid

   #159459

   Purpose

   Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   P2A-iCre
   

   Use

   Cre/Lox

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous TH

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-MSP1D1delltaH5

   Plasmid

   #71714

   Purpose

   Membrane scaffold protein (MSP), helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30-2-GPD1L

   Plasmid

   #83912

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a-dCas9-KRAB-GFP backbone

   Plasmid

   #194281

   Purpose

   Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL1_39

    Plasmid

    #199046

    Purpose

    Level 1 plasmid, barcoded ORI

    Depositor

    Cultivarium Tools, Charlie Gilbert, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pSa ORI plus NGS barcode 16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pdpy-30-miniCAFE

    Plasmid

    #170115

    Purpose

    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    Dpy-30

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti sfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164478

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-MVK

    Plasmid

    #23703

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MVK (MVK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pDONR223-ZNF280C

    Plasmid

    #88658

    Purpose

    Donor Vector containing ZNF280C transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF280C (ZNF280C Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Flag-NPM1c

    Plasmid

    #131813

    Purpose

    Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    This is the cytoplasmic mutant causing AML, where…

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTK_Cerulean_Nanotag_70_neg_Control

    Plasmid

    #130568

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLT3-ITD-MSCV-IRES-GFP

    Plasmid

    #184779

    Purpose

    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Activating internal tandem duplication (ITD) adde…

    Promoter

    MSCV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKER-mCardinal

    Plasmid

    #85976

    Purpose

    Expresses mCardinal from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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