We narrowed to 11,691 results for: NSI

1. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187922

   Purpose

   Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase acidic protein
   

   Tags

   3xFLAG

   Promoter

   CMV Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. trPAGmEos3.2

   Plasmid

   #203752

   Purpose

   Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trPAG (PAG1 Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pARF1(Q71L)-CFP

   Plasmid

   #11385

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 1 (ARF1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Changed Glutamine 71 to Leucine.

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ-EGFP:Ajuba

   Plasmid

   #108229

   Purpose

   Lentiviral vector for Tet-inducible Ajuba expression

   Depositor

   Kenneth Irvine

   Article

   Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

   Insert

   AJUBA (AJUBA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Tet/ON Minimal CMV promoter

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Cyto-ExRai-CKAR2

   Plasmid

   #236097

   Purpose

   Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   ExRai-CKAR2-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2 GFP-GSK3-MAPK

   Plasmid

   #29689

   Purpose

   GFP reporter protein fused to three GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site primed by MAPK
   

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site

   Promoter

   SP6

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB163

   Plasmid

   #37388

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   Dynactin 2 (DCTN2 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Mutation

   A7P

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pARF1(T31N)-CFP

   Plasmid

   #11384

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 1 (ARF1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 31 to Asparagine.

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Integrin beta1-tev-BHCys

   Plasmid

   #27314

   Depositor

   Timothy Springer

   Article

   Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

   Insert

   integrin beta1 (ITGB1 Human)
   

   Expression

   Mammalian

   Mutation

   integrin beta1(1-708)+GG+TEV+GGKN+BHCys

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pARF1-CFP

    Plasmid

    #11381

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCTCON2 Aga2P-HRP

    Plasmid

    #73152

    Purpose

    Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Aga2P-HRP
    

    Tags

    HA tag and myc tag

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR_zeo_sgNT135

    Plasmid

    #183181

    Purpose

    pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LCV2 control

    Plasmid

    #217443

    Purpose

    Lentiviral vector expressing Cas9 without a targeting sgRNA

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

    Plasmid

    #128608

    Purpose

    Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    36xMashTag
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF.DEST51-mScarlet

    Plasmid

    #207960

    Purpose

    Vector for overexpression of the mScarlet fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mScarlet
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET_RGG-GFP-RGG

    Plasmid

    #124948

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Benjamin Schuster, Matthew Good, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    EGFP and His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3_cAMPFIRE-L-R279E

    Plasmid

    #182282

    Purpose

    Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS_cAMPFIRE-L-R279E

    Plasmid

    #182286

    Purpose

    Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGB 35S:Luciferase:Tnos (GB0110)

    Plasmid

    #68216

    Purpose

    Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xFLAG-SENP7

    Plasmid

    #42886

    Depositor

    Edward Yeh

    Article

    Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

    Insert

    SENP7 (SENP7 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    T102A, R969C, L996S

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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