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Anti-Mitofusin-2 [N153/5.1R]

Plasmid

#114547

Purpose

Mammalian Expression Plasmid of anti-Mitofusin-2 (Mouse). Derived from hybridoma N153/5.1.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mitofusin-2 (Mus musculus) recombinant mouse monoclonal antibody (Mfn2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-p53DN-T2A-copGFP

Plasmid

#109229

Purpose

For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

Depositor

Martine Roussel

Publication

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

dominant negative p53 (Trp53 Mouse)

Tags

copGFP

Expression

Mammalian

Mutation

dominant negative p53

Promoter

CAGGS

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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fck-Jaws-KGC-GFP-ER2

Plasmid

#65012

Purpose

Lentivirus-mediated expression of Jaws under the CaMKII promoter.

Depositor

Edward Boyden

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

Lentiviral

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA-FRT-FAK10

Plasmid

#59119

Purpose

FAK fret sensor with 10 nm helix

Depositor

Sivaraj Sivaramakrishnan

Publication

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-RPS6KA2

Plasmid

#23530

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA2 (RPS6KA2 Human)

Use

Gateway Cloning

Mutation

C108T, A620G,2MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3 PBD wt (Nigg AH12)

Plasmid

#41162

Depositor

Erich Nigg

Publication

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PBD (PLK1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603)

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
mILK-EFGP

Plasmid

#176896

Purpose

mouse ILK cloned into pEGFP-N1

Depositor

Sara Wickström

Publication

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CMV500 A-SREBP1

Plasmid

#33357

Depositor

Charles Vinson

Publication

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SREBP1 (Srebf1 Cricetulus griseus (hamster))

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MMLV-RT(dRH) for Split-PE (pEH50)

Plasmid

#190106

Purpose

Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

Depositor

Keith Joung

Publication

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT(delta RNAseH)

Tags

bpNLS

Expression

Mammalian

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b

Plasmid

#182276

Purpose

Expresses recombinant BrCas12b

Depositor

Piyush Jain

Publication

Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis, T7, Thrombin site

Expression

Bacterial

Promoter

T7

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLIB-GST-3C-CDK1

Plasmid

#177011

Purpose

Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

Depositor

Andrea Musacchio

Publication

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CDK1 (CDK1 Human)

Tags

GST-3C

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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AiP1002-pAAV-mscRE16-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163486

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP1002 - pAAV-AiE2016m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1

Plasmid

#135503

Purpose

Mammalian expression of myc-tagged HLA-A*01:01

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

Plasmid

#124903

Purpose

Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

Depositor

Markus Covert

Publication

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

p65 promoter- p65 CDS-mRuby2

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-WT

Plasmid

#122690

Purpose

Express His-tagged PKM2 (wild type) protein in bacteria.

Depositor

Wen-Ching Wang

Publication

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

Plasmid

#71216

Purpose

dCas9 fused to human DNMT3A catalytic domain

Depositor

Yonglun Luo

Publication

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A catalytic domain

Use

Lentiviral

Tags

dSpCas9-DNMT3A

Expression

Mammalian

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Publication

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-TGFBR3

Plasmid

#23478

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TGFBR3 (TGFBR3 Human)

Use

Gateway Cloning

Mutation

C44T, 1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcmv3-IRG1-HA

Plasmid

#198181

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1)

Depositor

Yun Zhao

Publication

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

HA tag is added to C-terminal of target protein

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-Rbfox2-rABE-Flag

Plasmid

#191384

Purpose

Doxycycline inducible expression of Rbfox2-rABE

Depositor

Stephen Floor

Publication

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

Rbfox2-rABE (RBFOX2 Synthetic, Human)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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