We narrowed to 6,088 results for: pcDNA 3.1

1. mouse Drp1(K38A)

   Plasmid

   #26049

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Drp1 (Dnm1l Mouse)
   

   Expression

   Mammalian

   Mutation

   K38A GTPase mutant

   Available Since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pAB1679 CMV-HIVNES-GS-Cas13bt3

   Plasmid

   #176317

   Purpose

   CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Insert

   human codon optimized Cas13bt3
   

   Use

   CRISPR

   Tags

   HIV NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. KRas FAK biosensor

   Plasmid

   #78301

   Purpose

   FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   KRas FAK biosensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pHLuc (Antares-SEPLuc C1A4E+6)

   Plasmid

   #141089

   Purpose

   final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hHSF1 K80Q

   Plasmid

   #118351

   Purpose

   Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

   Depositor

   Lea Sistonen

   Article

   Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

   Insert

   hHSF1 (HSF1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K80Q

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTOPO-MCL1

   Plasmid

   #21605

   Depositor

   Ulrich Maurer

   Article

   Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

   Insert

   MCL-1 (MCL1 Human)
   

   Expression

   Mammalian

   Mutation

   Insert ends with a STOP codon, no V5-His-tag.

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

7. Myc-Bnip3FL

   Plasmid

   #100796

   Purpose

   Mammalian expression of Bnip3 full length splice variant

   Depositor

   Joseph Gordon

   Article

   Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

   Insert

   mus Bnip3 (Bnip3 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Opto-casp9_AtCRY2-PHR-casp9

   Plasmid

   #131221

   Purpose

   Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   Opto-casp9 (AtCRY2-PHR-casp9)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. VfAU1-LOV_P2A_casp9

   Plasmid

   #131224

   Purpose

   Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   VfAU1-LOV-HA_P2A_myc-casp9
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Arl6ip1-mEmerald

    Plasmid

    #186965

    Purpose

    Express Arl6ip1 with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    ARL6IP1 (ARL6IP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Actin Binding Domain Tension Sensor

    Plasmid

    #215368

    Purpose

    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tension Sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. NLS-dCas9-trCIB1

    Plasmid

    #64119

    Purpose

    Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    dCas9-trCIB1 fusion
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    dCas9 (D10A H840A, catalytically inactive), trCIB…

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-LiMETER-v1

    Plasmid

    #113933

    Purpose

    Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    LOV2 and Rit polybasic domain (PB)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-mito-CAR-GECO1

    Plasmid

    #46022

    Purpose

    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    CAR-GECO1
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

15. Mfn2(K109A)-16xmyc

    Plasmid

    #26051

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn2 (Mfn2 Mouse)
    

    Tags

    16xMyc

    Expression

    Mammalian

    Mutation

    K109A GTPase mutant

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

16. IDE-2Strep

    Plasmid

    #210355

    Purpose

    Expresses IDE fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    IDE (IDE Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid

    #227245

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1α and pTRE3G

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CoV2-N-T205I

    Plasmid

    #177954

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (T205I) (N )
    

    Expression

    Mammalian

    Mutation

    T205I

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY31

    Plasmid

    #84746

    Purpose

    Expresses huLbCpf1-P2A-puro and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Expression

    Insect

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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