We narrowed to 22,280 results for: fus
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Plasmid#175017PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertOK-HEFwtED
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)
Plasmid#108195PurposeExpresses PdR-PCNA3(R112C/T180C) fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C) fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_chr13_102010574-102010650
Plasmid#81213PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:FRB:nls:VPR
Plasmid#135987PurposeExpresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertFRB domain fused to the VPR activation domain
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch12_62418577-62418652
Plasmid#81212PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-Flex-rev-GFPFANACfullWT
Plasmid#126551PurposeAAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channelDepositorInserteGFP N-terminal fused to FMRFamide gate sodium channel
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP TSS/AAA mCherry
Plasmid#45234PurposeTarget INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
UseTagsCENP-B DBD, DNA binding domain of CENP-B and mChe…ExpressionMammalianMutationINCENP C-terminal TSS mutated to AAAPromotert7Available sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Flag-mIL-17RA/CFP.Hygro
Plasmid#46861Purposeencodes Flag-tagged mouse IL-17RA fused to Cyan Fl. ProteinDepositorInsertIL-17RA-CFP
UseTagsFlag/IL-3 signal sequence and fused to CFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS
Plasmid#69223PurposeExpresses R1335K mutant SpCas9 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationArginine 1335 is changed to LysinePromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-L7-6-DD-T6B-MUT-YFP
Plasmid#235146PurposeExpresses the inducible mutant T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoterDepositorInsertDHFR-fused mutant T6B peptide
UseAAVTagsExpressionMutation5 tryptophan residues of T6B-WT mutatedPromoterL7-6Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG022
Plasmid#231306PurposeVector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpRDepositorInsertBEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b Ag43 160N 6H mCherry-R5 AmpR
Plasmid#225141PurposeRecombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 160N, mCherry
UseTagsAg43 protein fused to silaffin-R5 and mCherryExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGG-PIP62
Plasmid#196800PurposeContains EDS1 promoterDepositorInsertENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1 Mustard Weed)
UseGreengate (gg) entry vectorTagsExpressionMutation151 G->C and 572 A->TPromoterAvailable sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
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