We narrowed to 10,893 results for: ENA;

1. PA-mCherry1-LaminB1-10

   Plasmid

   #57186

   Purpose

   Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Polymerase expression - pCMV-B103 (LM2907)

   Plasmid

   #208968

   Purpose

   Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC_C_H31

   Plasmid

   #111666

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   H31 (H3C1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pASK-mSAN1-Streptag

   Plasmid

   #117165

   Purpose

   Expresses Strep-tagged murine SAN1 nuclease in bacteria

   Depositor

   Ian Macara

   Article

   Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

   Insert

   mSAN1 (Fam120b Mouse)
   

   Use

   Tags

   Streptag

   Expression

   Bacterial

   Mutation

   Promoter

   tetR/tetA

   Available Since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

   Plasmid

   #105037

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nprl2 (Nprl2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

   Plasmid

   #105038

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nprl2 (Nprl2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PA-mCherry1-ER-5

   Plasmid

   #57174

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

   Insert

   ER (CALR Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

8. MDH1-miR-31SM-PGK-GFP-Blast

   Plasmid

   #31516

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-31 (Mir31 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   seed mutant control

   Promoter

   Available Since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-HA-VprBP (C590)

   Plasmid

   #133589

   Purpose

   Expresses human VprBP C590 mutant in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   Vpr (HIV-1) binding protein (DCAF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C590 mutation of human VprBP lacking the N-termin…

   Promoter

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. dmBACCS2VL-IRES-dOrai-IRES-mCherry

    Plasmid

    #72898

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80056

    Purpose

    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    flk1

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE

    Plasmid

    #192161

    Purpose

    AAV-CRISPRa base vector

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LSB-hsa-miR-138-5p

    Plasmid

    #103233

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-138-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-138-5p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC_C_HSP7C

    Plasmid

    #111667

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    HSP7C (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid

    #173866

    Purpose

    Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. dmBACCS2NS-IRES-dOrai-IRES-mCherry

    Plasmid

    #72896

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]

    Plasmid

    #184380

    Purpose

    The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoter

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSB-hsa-miR-19b-3p

    Plasmid

    #103327

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-19b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-19b-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P1_Firre_1.49_pDONR221

    Plasmid

    #195204

    Purpose

    Plasmid with pDONR221 backbone containing Firre 1.49 clone. Kanamycin resistance. Gateway cloning system.

    Depositor

    John Rinn

    Article

    Hacisuleyman et al Nat Struct Mol Biol. 2014 Feb;21(2):198-

    Insert

    mFirre 1.49 Clone
    

    Use

    Gateway cloning intermediate

    Tags

    Expression

    Mutation

    Only exons

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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