We narrowed to 2,784 results for: aen

1. pdCas9-M-G6

   Plasmid

   #73437

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor G6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZ194

   Plasmid

   #163640

   Purpose

   rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Synthetic, Nematode)
   

   Use

   CRISPR

   Tags

   2xmKate2 and GFP

   Expression

   Worm

   Mutation

   Promoter

   rps-27

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-M-3A2

   Plasmid

   #73435

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 3A2 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.27_eSOX17

   Plasmid

   #195498

   Purpose

   Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   Full distal endoderm enhancer region of SOX17 (SOX17 Human)
   

   Use

   Tags

   minP_luc2-hPEST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.27_eSOX17.2

   Plasmid

   #195499

   Purpose

   Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   Core distal endoderm enhancer region of SOX17 (SOX17 Human)
   

   Use

   Tags

   minP_luc2-hPEST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-M-4A6

   Plasmid

   #73434

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 4A6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCC336

   Plasmid

   #110167

   Purpose

   miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   Phospho-acceptor sites mutated: S43E, T55E, T62E.…

   Promoter

   lin-31 promoter/enhancer

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCC335

   Plasmid

   #110166

   Purpose

   miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   Phospho-acceptor sites mutated: S43A, T55A, T62A.…

   Promoter

   lin-31 promoter/enhancer

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pv6_MECP2_MBD-R106W

   Plasmid

   #179408

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MECP2_MBD(R106W) (Mecp2 Mouse)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   MBD R106W

   Promoter

   CAGGS

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAWK61

    Plasmid

    #163642

    Purpose

    rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Human, Nematode)
    

    Use

    CRISPR

    Tags

    2xmKate2 and GFP

    Expression

    Worm

    Mutation

    Promoter

    rps-27

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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