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pGLOW39

Plasmid

#173068

Purpose

mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

mScarlet-I-C1^SEC^3xMyc

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mScarlet

Expression

Worm

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-mCherry

Plasmid

#140306

Purpose

Expresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X FL

Expression

Mammalian

Mutation

contains only the SH4 and UD regions fused to mCh…

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pTD79

Plasmid

#172900

Purpose

Neuronal promoter rgef-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-S32

Plasmid

#140303

Purpose

Expresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

lacks the KD region

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-S32-KD

Plasmid

#140299

Purpose

Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

lacks the UD region

Promoter

CMV

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S32-KD

Plasmid

#140311

Purpose

Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Contains only the SH3, SH2, and KD regions

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBC2T-actin

Plasmid

#108968

Purpose

expresses BC2-tag-actin in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

beta-actin (ACTB Human)

Tags

BC2-tag

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTD80

Plasmid

#172901

Purpose

Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU

Plasmid

#179415

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7.Chromo/TAF3.PHD-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

Plasmid

#194897

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_WT
U6-crRNA-scramble

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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