We narrowed to 18,122 results for: URE

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Ppst93-MCRaeo-NB

Plasmid

#192764

Purpose

Expresses NB-tagged MCRaeo in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ppst93-MCRaeo-NG

Plasmid

#192763

Purpose

Expresses NG-tagged MCRaeo in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR

Plasmid

#189943

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-tagBFP

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP7_rpoA-U

Plasmid

#190974

Purpose

Test the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP7

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2-E67A_rpoA-U

Plasmid

#190959

Purpose

Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-A66S_rpoA-C

Plasmid

#190968

Purpose

Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP5_rpoA-U

Plasmid

#190985

Purpose

Test the U-to-C editing activity of KP5 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP5

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP1_rpoA-U

Plasmid

#190955

Purpose

Test the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2-A66S_rpoA-U

Plasmid

#190957

Purpose

Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3-A66S_rpoA-U

Plasmid

#190961

Purpose

Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP7_rpoA-U

Plasmid

#190994

Purpose

Test the U-to-C editing activity of KP7 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP7

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP3-E67A_rpoA-U

Plasmid

#190963

Purpose

Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP4_rpoA-U

Plasmid

#190964

Purpose

Test the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP4

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP6-E67A_rpoA-C

Plasmid

#190972

Purpose

Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP4_rpoA-U

Plasmid

#190984

Purpose

Test the U-to-C editing activity of KP4 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP4

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP5_rpoA-U

Plasmid

#190965

Purpose

Test the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP5

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr-CM

Plasmid

#188585

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler Catalytic mutant

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[N1-383]

Plasmid

#188586

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler N-terminal domain

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625]

Plasmid

#188587

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler C-terminal domain

Tags

FLAG-Myc

Expression

Insect

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBXPHM3-NB-D6

Plasmid

#167989

Purpose

expression of a nanobody against rat PepT2 as a n terminal MBP fusion

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

NanobodyD6

Tags

His-MBP

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2369-Tier1-PhCMV_KRAB

Plasmid

#169571

Purpose

Tier-1 vector encoding PhCMV-driven KRAB transsilencer domain (PhCMV-KRAB-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Kruppel associated box domain

Expression

Mammalian

Promoter

PhCMV

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.22

Plasmid

#183566

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 22

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.13

Plasmid

#183576

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 13

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MTOR

Plasmid

#183311

Purpose

All-in-One CRISPRko system with a guide RNA that targets MTOR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MTOR

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_BRAF

Plasmid

#183273

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRAF gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRAF

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2341_Tier3(SB)-Puro

Plasmid

#169639

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pgTS40

Plasmid

#169630

Purpose

pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

U6-driven gRNA expression and PCAG-driven SpCas9 expression

Expression

Mammalian

Promoter

U6 / PCAG

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1041

Plasmid

#169619

Purpose

Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc and mTagBFP2

Expression

Mammalian

Promoter

PPGK

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH228-Tier1-OVanO2-PCMVmin2-SEAP

Plasmid

#169564

Purpose

Tier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

vanillic acid-controlled SEAP production

Expression

Mammalian

Promoter

VanO2-PCMVmin

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-F>E

Plasmid

#185508

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

F>E

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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