We narrowed to 7,669 results for: CCH

1. Sc NybbR eRF3∆165N (WT)

   Plasmid

   #175419

   Purpose

   Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

   Depositor

   Joseph Puglisi

   Article

   Lawson et al Science. 2021 Aug 20;373(6557):876-882.

   Insert

   eRF3 (SUP35 Budding Yeast)
   

   Tags

   His-MBP-TEV and ybbR

   Expression

   Bacterial

   Mutation

   ∆165N

   Promoter

   T7

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYES2.1-cerMET5

   Plasmid

   #107437

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET5 (MET5 Budding Yeast)
   

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

   Promoter

   GAL1

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EC0_2_VP64

   Plasmid

   #163714

   Purpose

   Plasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   VP64 + SV40 NLS
   

   Use

   Synthetic Biology; Moclo vector level 0 (type 4a)

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS303 Bt δCOP

   Plasmid

   #73871

   Purpose

   Bos taurus Arcn1

   Depositor

   Blanche Schwappach

   Article

   Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Ret2 Promoter- COPD
   

   Expression

   Yeast

   Promoter

   Ret2 Promoter

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHB4

   Plasmid

   #53462

   Purpose

   This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

   Depositor

   Elizabeth Conibear

   Article

   Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

   Insert

   yeGFP-Snc1-Suc2
   

   Expression

   Yeast

   Promoter

   ADH

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDYSCKO

   Plasmid

   #120263

   Purpose

   Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

   Depositor

   Christian Landry

   Article

   Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

   Insert

   DYSCKO cassette
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   SNR52

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS426-TUB1-internal6xHis

   Plasmid

   #60390

   Purpose

   Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4)

   Promoter

   GAL

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pILGFP3A2 (2)

   Plasmid

   #185852

   Purpose

   Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PMV-ycrtI

   Plasmid

   #65342

   Purpose

   systhesis of crtI gene in the beta-carotene pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   ycrtI
   

   Use

   Synthetic Biology

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3_4xUAS_CP-candidate_luc+

    Plasmid

    #125154

    Purpose

    STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Luciferase

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. POM42-mCitrine

    Plasmid

    #69854

    Purpose

    N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCitrine minus start and stop codon
    

    Expression

    Yeast

    Available Since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJCSUP35(254-684)

    Plasmid

    #1092

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids 254 - 684 of SUP35

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pETM6-H9-mCherry

    Plasmid

    #66532

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H9

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H9

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. PMV-ycrtYB

    Plasmid

    #65343

    Purpose

    systhesis of crtYB gene in the beta-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    ycrtYB
    

    Use

    Synthetic Biology

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAX_CAGGS_CAS9_VPR_EYFP

    Plasmid

    #167890

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAct:MS2-p65-HSF

    Plasmid

    #78902

    Purpose

    Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2-p65-HSF
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDownstream1Lox

    Plasmid

    #164574

    Purpose

    Integration of 1 LoxN site in the genome of P. berghei

    Depositor

    Olivier Silvie

    Type

    Empty backbone

    Use

    Cre/Lox

    Promoter

    Bidirectional PbEF1alpha promoter (PBANKA_1133300…

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS416 Gal-RNQ1 153-405-YFP

    Plasmid

    #18689

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 153-405
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Prion domain of Rnq1

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

19. NAX_CAGGS_CAS9_m4_EYFP

    Plasmid

    #167866

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PMV-ycrtE

    Plasmid

    #65341

    Purpose

    systhesis of crtE gene in the beta-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    ycrtE
    

    Use

    Synthetic Biology

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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