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pCAG-Mac_Q139D_D150N_E258V-HaloTag

Plasmid

#129259

Purpose

Express Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Mac_Q139D_D150N_E258V-HaloTag

Expression

Mammalian

Mutation

Q139D,D150N,E258V

Promoter

CAG

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag

Plasmid

#129258

Purpose

Express QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

QuaAr3_Q95D_H106N_E214V-HaloTag

Expression

Mammalian

Mutation

Q95D, H106N, E214V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Scarlet-Borcs7-Q87X

Plasmid

#118751

Purpose

Expresses scarlet tagged Borcs7-Q87X

Depositor

Beverly Koller

Insert

Borcs7-Q87X cDNA (2010012O05Rik Mouse)

Tags

mScarlet

Expression

Mammalian

Mutation

Q87X

Promoter

CAG

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-ePsuACR-mRuby2-ST

Plasmid

#109133

Purpose

Anion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ePsuACR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-eiC++-mRuby2-ST

Plasmid

#109132

Purpose

Artificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eiC++-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-CsChR-mRuby2-ST

Plasmid

#109130

Purpose

Cation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

CsChR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-mCherry-FHA1-subT391A-mCherry

Plasmid

#104462

Purpose

Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

Depositor

Bernardo Sabatini

Article

Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

Insert

mCherry-subT391A-FHA1-mCherry

Use

AAV

Tags

mCherry

Promoter

CAG

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-Avr-HPRT-RIGHT

Plasmid

#107272

Purpose

Expresses TALEN (right) targeting HPRT exon3

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

HPRT1_B Avr-TALEN (right)

Use

TALEN

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-Avr-HPRT-LEFT

Plasmid

#107271

Purpose

Expresses TALEN (left) targeting HPRT exon3

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

HPRT1_B Avr-TALEN (left)

Use

TALEN

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPickles-2.31

Plasmid

#92336

Purpose

FRET-based biosensor for the measurement of BCR-ABL activity

Depositor

Yusuke Ohba

Article

Mizutani et al Clin Cancer Res. 2010 Aug 1;16(15):3964-

Insert

Pickles-2.31

Expression

Mammalian

Promoter

CAG

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

Plasmid

#104570

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x3)

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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RV-cag-Dio-Kif5c-2A-tdt

Plasmid

#87668

Purpose

retroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Kif5C-P2A-mTdt (Kif5c Rat)

Use

Retroviral

Tags

palmitylation

Mutation

motor domain aa1-560 present, V40A, E125V (see de…

Promoter

CAG

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pWN10042

Plasmid

#89052

Purpose

GF-ori-FP plasmid for GFxFP assay

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

EGFP part 1
EGFP part 2

Use

CRISPR

Expression

Mammalian

Promoter

CAG and none

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Mbd3b-pA-pgk-bsd

Plasmid

#74920

Purpose

Mammalian expression of Mbd3b

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Mnd3b (Mbd3 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-aVent1-pgk-hph

Plasmid

#74916

Purpose

Mammalian expresion of aVent1

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Vent1

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-aBsx-pgk-hph

Plasmid

#74913

Purpose

Mammalian expression of aBsx

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Bsx

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-zNanogHD-pgk-hph

Plasmid

#74910

Purpose

Mammalian expression of zNanog Homeodomain

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-mNanogHD-pgk-hph

Plasmid

#74909

Purpose

Mammalian expression of mNanog Homeodomain

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Mbd3c-pA-pgk-bsd

Plasmid

#74921

Purpose

Mammalian expression of Mbd3c

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Mbd3c (Mbd3 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
CAG-mCherry-RORbshRNA2

Plasmid

#73984

Purpose

RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse RORb gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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