We narrowed to 13,725 results for: cas9

1. pHU6_Ch12_62418577-gRNA-rev

   Plasmid

   #81217

   Purpose

   pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   hU6 expression of gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHU6_Ch12_62418577-gRNA-for

   Plasmid

   #81216

   Purpose

   pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch12 reporter

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   hU6 expression of gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKF251

   Plasmid

   #52532

   Purpose

   Double-GFP reporter for DNA cleavage

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   Double-GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   ubiquitin

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCALNL-eGFP-Esp3I

   Plasmid

   #81226

   Purpose

   Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   Esp31-Neo-Esp31 deletion cassette upstream of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCBa

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB4780

   Plasmid

   #106143

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNRCreCNAT

   Plasmid

   #234430

   Purpose

   Picochlorum celeri CloNat insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal

   Depositor

   Matthew Posewitz

   Article

   Burch et al Front Microbiol. 2025 Jul 14;16:1588625.

   Insert

   Cre recombinase
   

   Use

   CRISPR, Cre/Lox, and Synthetic Biology ; Green al…

   Tags

   Expression

   Mutation

   Picochlorum celeri endoglucanase intron insertion

   Promoter

   P celeri nitrate reductase

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNRCreBle

   Plasmid

   #234431

   Purpose

   Picochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal

   Depositor

   Matthew Posewitz

   Article

   Burch et al Front Microbiol. 2025 Jul 14;16:1588625.

   Insert

   Cre recombinase
   

   Use

   CRISPR, Cre/Lox, and Synthetic Biology ; Green al…

   Tags

   Expression

   Mutation

   Picochlorum celeri endoglucanase intron insertion

   Promoter

   P celeri nitrate reductase

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHelper-CRISPRi-sgRNA

   Plasmid

   #221135

   Purpose

   Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with dCas9 handle
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119(SpeI)

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

   Plasmid

   #99696

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDM043

    Plasmid

    #216813

    Purpose

    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mScarlet3
    

    Use

    Tags

    VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDM052

    Plasmid

    #216814

    Purpose

    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mNeonGreen
    

    Use

    Tags

    VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDM070

    Plasmid

    #216816

    Purpose

    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mCardinal
    

    Use

    Tags

    VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWR91

    Plasmid

    #203915

    Purpose

    empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Insert

    CRF1 plus flanking (CRF1 S. stipitis (yeast))
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWR90

    Plasmid

    #203914

    Purpose

    empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Insert

    GSC2 plus flanking (GSC2 S. stipitis (yeast))
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWR89

    Plasmid

    #203423

    Purpose

    empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Insert

    EGC3 plus flanking (EGC3 S. stipitis (yeast))
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCL390

    Plasmid

    #219500

    Purpose

    Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
    

    Use

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-GAL10

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSK-Spsg

    Plasmid

    #214351

    Purpose

    Expresses cloning backbone for SpCas9 sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT120a17-sg-del

    Plasmid

    #214347

    Purpose

    Expresses pIII

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    gIII-xLuxAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJAT58

    Plasmid

    #204304

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. rtTA-sg-mGreenLantern332

    Plasmid

    #188483

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    PB_rtTA_BsmBI

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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