Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,129 results for: cas9 expression plasmid

Clear keywords
Showing: 5341 - 5360 of 6129 results

  3. pFA0057

    Plasmid
    #131784
    Purpose
    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer GFP cassette guide (gGFP) and 5' sgRNA
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131784-plasmid-map-sequence-id-258729

    Enlarge

  4. DBJS-p2.11

    Plasmid
    #246891
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for G3BP1 Nterm.
    Depositor
    David Bartel
    Insert
    G3BP1 Nterm Guide RNA 1 (G3BP1 Human)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246891-plasmid-map-sequence-id-490580

    Enlarge

  5. DBJS-p2.16

    Plasmid
    #246893
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for CAPRIN1 Nterm.
    Depositor
    David Bartel
    Insert
    Caprin1 Nterm Guide RNA 1 (CAPRIN1 Human)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246893-plasmid-map-sequence-id-490578

    Enlarge

  6. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

    Enlarge

  7. pINSPECT:NEAT1-v2_SigP:NLuc

    Plasmid
    #197268
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-v2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197268-plasmid-map-sequence-id-389661

    Enlarge

  8. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid
    #197267
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-total homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197267-plasmid-map-sequence-id-400583

    Enlarge

  13. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

    Enlarge

  18. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

    Enlarge

Showing: 5341 - 5360 of 6129 results