We narrowed to 32,001 results for: Eng

1. pNLF1-C_FBXW11:1-542

   Plasmid

   #210952

   Purpose

   Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   FBXW11:M1-R542 (FBXW11 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiT3.1-N_SKIC8:1-305

   Plasmid

   #211038

   Purpose

   Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   SKIC8:M1-I305 (SKIC8 Human)
   

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-mCherry-NLS

   Plasmid

   #165442

   Purpose

   Expresses nuclear mCherry from CMV promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

   Insert

   mCherry-NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

   Plasmid

   #168291

   Purpose

   optimized miniCMV (OminiCMV)

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   mCherry
   

   Expression

   Mammalian

   Promoter

   miniCMV

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-R2Tg_opt-3xFlag

   Plasmid

   #223251

   Purpose

   Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg protein_opt
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176268

   Purpose

   Expression of dCas12f-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCas12f
   

   Use

   Lentiviral

   Tags

   HA-VPR-mCherry

   Expression

   Mammalian

   Mutation

   D326A, D510A

   Promoter

   PGK

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1a-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223252

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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