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pFA6a-kanMX6-PGAL1-HBH

Plasmid

#26885

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag N-terminal

Use

Pcr tagging yeast

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-HBH

Plasmid

#26886

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag N-terminal

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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RPS6KA1 gRNA (BRDN0001145974)

Plasmid

#75497

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA1 (RPS6KA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PRKAA2ΔC

Plasmid

#30307

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α2 ΔC (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids Ser 527 to Arg 552

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
nPEX-LV

Plasmid

#12119

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Mutation

noncatalytic fragment of MMP2 amplified via PCR, …

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
Lifeact-3xmNG::URA3

Plasmid

#227595

Purpose

Integration vector for expressing Lifeact-3xmNeonGreen

Depositor

Bruce Goode

Article

Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

Insert

Lifeact-3xmNeonGreen::URA3

Tags

3xmNeonGreen

Expression

Yeast

Mutation

Truncated ABP140 (aa 1-17 only)

Promoter

Abp140

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACS131

Plasmid

#204596

Purpose

Histone H3.1K27M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 K27M (H3C1 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

Plasmid

#199360

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOEu-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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EC64422

Plasmid

#191768

Purpose

CRISPR mutagenesis in Medicago truncatula

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

Insert

SpCas9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf9c

Plasmid

#158379

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf9c

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGDP4 tet(A)

Plasmid

#112887

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

tet(A)

Tags

6xHis

Expression

Bacterial

Mutation

Sequence was codon optimized for E. coli K12 usin…

Promoter

Plac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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sh-SRF

Plasmid

#100797

Purpose

expression of shRNA targeting SRF

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

Insert

rat SRF

Use

RNAi

Promoter

H1

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB3042(gRNA X-4)

Plasmid

#73284

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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T-STOP sgRNA-1

Plasmid

#83346

Purpose

The sgRNA at the stop codon of human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

T-STOP sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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shGLDC-DOX

Plasmid

#72883

Purpose

dox inducible knockdown

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

RNAi hairpin against GLDC

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only
pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSMP-Bmi1_2

Plasmid

#36353

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pCAGGS-hCD4-GCaMP2

Plasmid

#18932

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

hCD4-GCaMP2 (CD4 Human)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
p209 pCMV-cre-K

Plasmid

#8394

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

Modified 5' Cre

Use

Cre/Lox

Expression

Mammalian

Mutation

Modified 5' region of Cre

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pLV-UbC-Tet1c-dCas9

Plasmid

#232178

Purpose

Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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