We narrowed to 59,446 results for: cloning

1. pPOM013_P2_pENO101

   Plasmid

   #216438

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter eno101
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-CDK12-V5-TurboID_IDG-K

   Plasmid

   #135244

   Purpose

   Gateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK12-V5-TurboID (CDK12 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRATIO3267

   Plasmid

   #141312

   Purpose

   Gateway cloning compatible dual bioluminescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Tags

   gLUC and redLUC

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRATIO3212

   Plasmid

   #141311

   Purpose

   Gateway cloning compatible dual fluorescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Tags

   mScarlet-I and mVenus

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR - CaSR-EGFP

   Plasmid

   #191503

   Purpose

   A zebrafish CaSR-EGFP construct used for Gateway cloning.

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Zebrafish expression

   Promoter

   none

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH103

   Plasmid

   #128208

   Purpose

   crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAK005

   Plasmid

   #128218

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 5

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 5
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAK003

   Plasmid

   #128216

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 3

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 3
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAK004

   Plasmid

   #128217

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 4

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAK006

    Plasmid

    #128219

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 6

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 6
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-Fn-crRNA

    Plasmid

    #78958

    Purpose

    Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOGG010

    Plasmid

    #113984

    Purpose

    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    RK2 (IncP)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAK002

    Plasmid

    #128215

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 2

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 2
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p667-UBC-CDC42BPB-V5-miniTurbo_IDG-K

    Plasmid

    #198008

    Purpose

    Gateway destination clone of CDC42BPB (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDC42BPB-V5-miniTurbo (CDC42BPB Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO GFP DNAJB2a

    Plasmid

    #19497

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2a
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. BASIC_11_RBS34-GFP-B0015

    Plasmid

    #68145

    Purpose

    storage vector for BASIC part: RBS-GFP(ORF)

    Depositor

    Geoffrey Baldwin

    Article

    Storch et al ACS Synth Biol. 2015 Apr 16.

    Insert

    RBS-34-GFP-B0015
    

    Expression

    Bacterial

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-miniTurbo-V5-CDK17_IDG-K

    Plasmid

    #158174

    Purpose

    Gateway destination clone of CDK17 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK17 (CDK17 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. GGE367

    Plasmid

    #120736

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Antibiotic resistance marker for selection after transformation in Y. lipolytica (Marker_hph)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    Marker_hph
    

    Use

    Cloning/assembly plasmid

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p663-UBC-miniTurbo-V5-CAMKK2_IDG-K

    Plasmid

    #170577

    Purpose

    Gateway destination clone of CAMKK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CAMKK2 (CAMKK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOGG011

    Plasmid

    #113985

    Purpose

    Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    pBBR1
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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