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pCTD26-S>A2-9

Plasmid

#112825

Purpose

pSMF2 with all S to A mutations in only repeats 2-9.

Depositor

Stephen Fuchs

Article

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 2…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCTD26-S>A18-25

Plasmid

#112827

Purpose

pSMF2 with all S to A mutations in only repeats 18-25.

Depositor

Stephen Fuchs

Article

Babokhov et al G3 (Bethesda). 2018 May 4;8(5):1593-1601

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

pSMF2 with all S to A mutations in only repeats 1…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-D-recJD

Plasmid

#112784

Purpose

Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

recJD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-NFIB2ERt2

Plasmid

#112703

Purpose

Expression of NFIB2-ERT2 fusion protein

Depositor

Richard Gronostajski

Insert

Nuclear Factor I B2 (Nfib Mouse)

Use

Tags

ERT2 and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Adeno-miR-27b

Plasmid

#112539

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Article

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-27b (MIR27B Human)

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Rad C249

Plasmid

#11228

Depositor

Ronald Kahn

Article

Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9

Insert

Rad (RRAD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C249: deletion of amino acids 249-308

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD14

Plasmid

#112822

Purpose

pSMF2 with 14 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3

Plasmid

#112658

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

sfGFP-Glo3

Use

Tags

sfGFP

Expression

Yeast

Mutation

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pExp-CHis

Plasmid

#112559

Purpose

Used for expression of protein in E. coli as C-terminual 8His fusions

Depositor

Marko Hyvönen

Type

Empty backbone

Use

Tags

8His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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peAA.11

Plasmid

#112149

Purpose

"Vector AA"

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-Pars

Plasmid

#112779

Purpose

Promoter of ars operon. Repressed by ArsR, induced by arsenate or arsenite

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

Pars promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF331.1.0-gDNA

Plasmid

#112461

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF331

Depositor

Kevin White

Insert

ZNF331 (ZNF331 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-EHC

Plasmid

#112619

Purpose

expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEHC

Plasmid

#112615

Purpose

promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Promoterless vector, ready for promoter for mamma…

Tags

Emerald

Expression

Mammalian

Mutation

Promoter

No Promoter

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pmTHC

Plasmid

#112616

Purpose

promoterless expression plasmid driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-mTFP1 (H2BC3 Anthozoa, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Promoterless vector, ready for promoter for mamma…

Tags

mTeal (mTFP1)

Expression

Mammalian

Mutation

Promoter

No Promoter

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (wt)

Plasmid

#112799

Purpose

Expression of OCTN2

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Use

Tags

3 x Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-LSS-SGFP2

Plasmid

#112943

Purpose

Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Insert

LSS-GFP2 mCherry fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Use

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MCV-HF LTS220A/S239A

Plasmid

#112191

Purpose

Merkel cell polyomavirus molecular clone

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCV-HF LTS220A/S239A

Use

Tags

Expression

Mutation

T to G mutations at 3313 (nt) and 3370 (nt) of fu…

Promoter

None from vector. Insert contains native promoter…

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_hM3O

Plasmid

#112638

Purpose

Plasmid for PCR amplification of hM3O DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

hM3O

Use

Pcr template

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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