We narrowed to 7,152 results for: Ank;

1. Tol2-GgVCL/*TBM

   Plasmid

   #162790

   Purpose

   MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

   Depositor

   Laura Lafon-Hughes

   Article

   et al PeerJ

   Insert

   Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
   

   Tags

   IRES-GFP

   Expression

   Bacterial and Mammalian

   Mutation

   changed Gly 454 to Val

   Promoter

   beta actin

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. RNAPII_CTD27-52_N2A

   Plasmid

   #98677

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N1775A, N1782A

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. RNAPII_CTD27-52_del1961-1970

   Plasmid

   #98680

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   del1961-1970

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. RNAPII_CTD27-52_N2A_S1966C

   Plasmid

   #98682

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N1775A, N1782A, S1966C

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. RNAPII_CTD27-52_K5S_S1966C

   Plasmid

   #98683

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966C

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K1838S, K1859S, K1866S, K1873S, K1887S, S1966C

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-SpELL pNMT-TOPO

   Plasmid

   #15315

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

   Insert

   eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
   

   Tags

   FLAG

   Expression

   Yeast

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

7. FLAG-SpEAF pNMT-TOPO

   Plasmid

   #15316

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

   Insert

   ELL associated factor (eaf1 Fission Yeast)
   

   Tags

   FLAG

   Expression

   Yeast

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-SpELL pBacPAK8

   Plasmid

   #15317

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

   Insert

   eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
   

   Use

   Baculovirus construction

   Tags

   FLAG

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pECE-M2-PP1Cbeta

   Plasmid

   #31677

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)
   

   Tags

   M2

   Expression

   Mammalian

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pS648∙CsR

    Plasmid

    #197860

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pS148∙CsR

    Plasmid

    #197858

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Ap/Cb resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSH624-ssr

    Plasmid

    #199241

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    single-stranded DNA recombineering
    

    Expression

    Bacterial

    Promoter

    lacIq-Ptrc

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS548∙CsR

    Plasmid

    #197859

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Tc resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pS848∙CsR

    Plasmid

    #199240

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM7

    Plasmid

    #201213

    Purpose

    Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.

    Depositor

    Alex Nielsen

    Article

    Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1

    Inserts

    sfGFP
    Spo0A right flank
    Spo0A left flank
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    F11V, T13P, N39D, A179A

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM-U6-reRNA-acceptor-2xBbsI

    Plasmid

    #205633

    Purpose

    Cloning and U6-drive expression of ISDra2 TnpB reRNAs

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS316-RGR-GFP

    Plasmid

    #51056

    Purpose

    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEYFP-C1-Drp1

    Plasmid

    #45160

    Depositor

    Richard Youle

    Article

    Frank et al Dev Cell. 2001 Oct;1(4):515-25.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. CibN

    Plasmid

    #209671

    Purpose

    Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

    Depositor

    Sanjeevi Sivasankar

    Article

    Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Tags

    Split-TurboID-N and pmEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 NL

    Plasmid

    #113442

    Purpose

    NanoLuc control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    cmyc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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