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pAS3-rab-3P::AI::QF+hGR::SL2::tagBFP

Plasmid

#232607

Purpose

Pan-neuronal expression of chimeric protein 'QF+hGR' and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

QF+hGR

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::gur-3G

Plasmid

#232605

Purpose

Pan-neuronal expression of 'GUR-3' using a genomic fragment, regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-5xQUAS::Δpes-10P::AI::prdx-2G

Plasmid

#232614

Purpose

Expression of 'PRDX-2' on activation via 'QF+hGR' chimeric protein

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

PRDX-2 (prdx-2 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::lite-1G

Plasmid

#232606

Purpose

Pan-neuronal expression of 'LITE-1' using a genomic fragment, regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

LITE-1 (lite-1 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-T Dmel Or94b

Plasmid

#59691

Purpose

Probe for Drosophila melanogaster Or94b expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or94b (Or94b Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBS-SK(-) Dmel Or23a

Plasmid

#59644

Purpose

Probe for Drosophila melanogaster Or23a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or23a (Or23a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or33b

Plasmid

#59648

Purpose

Probe for Drosophila melanogaster Or33b expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or33b (Or33b Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBS-SK(-) Dmel Or43a

Plasmid

#59653

Purpose

Probe for Drosophila melanogaster Or43a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or43a (Or43a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or59a

Plasmid

#59664

Purpose

Probe for Drosophila melanogaster Or59a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or59a (Or59a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBS-SK(-) Dmel Or2a

Plasmid

#59635

Purpose

Probe for Drosophila melanogaster Or2a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or2a (Or2a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
tdTomato-N1

Plasmid

#54642

Purpose

Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMEDS

Plasmid

#236568

Purpose

Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.

Depositor

Shannon Moonah

Article

Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi

Inserts

CD/UPRT (negative selection)
NPT II (positive selection)

Tags

3x Myc and FLAG

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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CCR5-donor-MCS

Plasmid

#192269

Purpose

Donor template for CRISPR Genome Editing

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

MCS within donor template

Use

CRISPR

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLQ-KO-tgt50

Plasmid

#126578

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50

Use

CRISPR

Expression

Bacterial

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-T43NTR-TrxA-H10

Plasmid

#121677

Purpose

Bacterial expression of neurotensin receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

Insert

neurotensin receptor (Ntsr1 Rat)

Tags

TrxA-H10

Expression

Bacterial

Mutation

deleted amino acids 1-42

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
Or1a-Gal4

Plasmid

#63019

Purpose

To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4

Depositor

Leslie Vosshall

Article

Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

Insert

Drosophila melanogaster Or1a promoter

Tags

Drosophila ADH (aa's 1-18) fused to Yeast Ga…

Expression

Insect

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only
pBS-SK(-) Dmel Or22a

Plasmid

#59641

Purpose

Probe for Drosophila melanogaster Or22a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or22a (Or22a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
AAV-CAG-DIO-mAPX

Plasmid

#79908

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

mAPX

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-SK(-) Dmel Orco

Plasmid

#59680

Purpose

Probe for Drosophila melanogaster Orco expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Orco (Orco Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
Or30a-Gal4

Plasmid

#63027

Purpose

To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or30a, using the promoter sequences of the gene cloned 5' of Gal4

Depositor

Leslie Vosshall

Article

Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

Insert

Drosophila melanogaster Or30a promoter

Tags

Drosophila ADH (aa's 1-18) fused to Yeast Ga…

Expression

Insect

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only