We narrowed to 704 results for: transfection
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Plasmid#131838PurposeExpresses the last 50 amino acids of HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationThis is a deletion contruct of the cytoplasmic m…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1cOligodN243-C1
Plasmid#131847PurposeExpresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationThis is a deletion contruct of the cytoplasmic m…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1cNLSdN243-C1
Plasmid#131848PurposeExpresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationThis is a deletion contruct of the cytoplasmic m…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPMMLF1nes-C1
Plasmid#131849PurposeExpresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationThis is a control deletion contruct of the cytop…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-2-3CL
Plasmid#160278PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TriEx-4 DHX36
Plasmid#68368PurposeExpresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.DepositorInsertDHX36 (DHX36 Human)
UseTagsHistidineExpressionBacterial, Insect, and Mamm…MutationPromoterCMV ie enhancer/promoterAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rac1(P29S)
Plasmid#224280PurposeCell transfection and expression of Rac1 (P29S)DepositorInsertRac1 (RAC1 Human)
UseTagsExpressionMammalianMutationP29SPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rac1(WT)
Plasmid#224278PurposeCell transfection and expression of Rac1DepositorInsertRac1 (RAC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424TagsExpressionMutationThe conserved GDD in the NS5 polymerase gene muta…PromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rac1(G12C)
Plasmid#224279PurposeCell transfection and expression of Rac1 (G12C)DepositorInsertRac1 (RAC1 Human)
UseTagsExpressionMammalianMutationG12CPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-ACTB
Plasmid#227320PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)DepositorInsertACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-jYCaMP1
Plasmid#135423PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-2-3CL-C145A
Plasmid#160279PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC145APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-150_175-470)
Plasmid#170537PurposeExpresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationdeleted amino acids 151-175PromoterCAG promoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-IBV-3CL
Plasmid#160288PurposeAllows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-HCoV-NL63-3CL
Plasmid#160286PurposeAllows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-Bat-CoV-HKU9-3CL
Plasmid#160284PurposeAllows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-jYCaMP1s
Plasmid#135424PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variantDepositorHas ServiceAAV1InsertjYCaMP1s
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-3CL
Plasmid#160280PurposeAllows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-jYCaMP1
Plasmid#135422PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only