We narrowed to 11,691 results for: NSI

1. pDT-sgRNA-XMAS-1x

   Plasmid

   #164413

   Purpose

   Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-miRE_shBRD9_561-SV40-GFP

   Plasmid

   #75139

   Purpose

   Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   shBRD9_561
   

   Use

   Retroviral

   Available Since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pFnCas12a_NT

   Plasmid

   #169838

   Purpose

   Expresses FnCas12a and guide RNA in Bacteria

   Depositor

   John van der Oost

   Article

   Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

   Insert

   FnCas12a
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pXynA

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mt deltaN20 TFB2MdeltaC3

   Plasmid

   #205062

   Purpose

   TFB2M protein expression

   Depositor

   Smita Patel

   Article

   Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

   Insert

   TFB2M (TFB2M Human)
   

   Expression

   Bacterial

   Mutation

   Lacks N-terminal 20 aa and C-terminal 3 aa

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2 CA-LRP6-GFP

   Plasmid

   #29682

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   LDL-receptor related protein 6 (LRP6 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   extracellular region deleted

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. mCherry-Incenp wt

   Plasmid

   #108487

   Purpose

   expression of vsv-INCENP wt-mCherry

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   INCENP (INCENP Human)
   

   Tags

   VSV and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET_RGG-RGG-RGG

   Plasmid

   #124938

   Purpose

   Expression of RGG-RGG-RGG

   Depositor

   Benjamin Schuster, Daniel Hammer, Matthew Good

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIBA3-SpyCatcher-sfGFP

   Plasmid

   #107420

   Purpose

   For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

   Depositor

   Jack Leo

   Article

   Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

   Insert

   SpyCatcher
   

   Tags

   His tag, StrepII, and superfolder GFP

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDR-GFPuniv

   Plasmid

   #46085

   Purpose

   In vivo recombination assay fluorescent reporter

   Depositor

   Raymond Monnat

   Article

   Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

   Insert

   DR-GFPuniv reporter
   

   Use

   Recombination reporter plasmid

   Expression

   Mammalian

   Mutation

   E5G and K163R mutations in EGFP relative to wild-…

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_FLAG-Mma PylRS

    Plasmid

    #154762

    Purpose

    plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag

    Plasmid

    #191536

    Purpose

    Overexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dMisCas13b-SNAPf-NLS-3xFlag
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-IRES-puro-NLS-dMisCas13b-EGFP-NLS

    Plasmid

    #191359

    Purpose

    Overexpression of NLS-dMisCas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dMisCas13b-EGFP-NLS
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_TEC-1/1

    Plasmid

    #91252

    Purpose

    Protein expression and purification of human SH3 domain construct TEC-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TEC-1/1 (TEC Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-mFzd2

    Plasmid

    #42254

    Depositor

    Jeremy Nathans, Chris Garcia

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 2 (Fzd2 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-OSH2-2xPH

    Plasmid

    #161987

    Purpose

    Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

    Depositor

    David Bryant, Tamas Balla

    Article

    Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

    Insert

    OSH2 (OSH2 Budding Yeast)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. TDP43 NOTAG2

    Plasmid

    #28207

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    aa86-414 only

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pHis-hMTMR1

    Plasmid

    #100723

    Purpose

    Expression of human MTMR1 PH-PTP domain

    Depositor

    Byung Il Lee

    Article

    Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

    Insert

    MTMR1 (MTMR1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 95-607

    Promoter

    T7

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-CALPAIN-7-mCherry

    Plasmid

    #180652

    Purpose

    Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GCACCCAUACCUUUACAUU

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-RAB-EKARev(T/A)

    Plasmid

    #118475

    Purpose

    Negative-control mutant for RAB-EKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-ExRai-AktAR(T/A)

    Plasmid

    #118463

    Purpose

    Negative-control mutant for ExRai-AktAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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