We narrowed to 7,101 results for: tev

1. pET28a Cdk2ap1CAN-MutTER

   Plasmid

   #178033

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   Changed T(108)A, E(109)A, R(110)A

   Promoter

   T7LacI

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a Cdk2ap1ΔN (MT2B2)

   Plasmid

   #178034

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted

   Promoter

   T7LacI

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSNA-c029

   Plasmid

   #175471

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   L281R, H288A

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SYKA-c022

   Plasmid

   #175490

   Purpose

   Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

   Depositor

   Opher Gileadi

   Article

   Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

   Insert

   SYK (SYK Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSNA-c028

   Plasmid

   #175470

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   H288A

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSNA-c021

   Plasmid

   #175468

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   L281R

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSNA-c001

   Plasmid

   #175466

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CEA-N domain residues 1-132 (CEACAM5)-pET30b

   Plasmid

   #174693

   Purpose

   Plasmid expressing CEA-N domain

   Depositor

   Jean Gariepy

   Article

   Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

   Insert

   CEA-N Domain
   

   Use

   Tags

   TEV Cleavage Site and hexahistidine (HIS) tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 Promoter

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T3-GST-PolB(K206A/K244A/L301R/V303R/V306R)

   Plasmid

   #177138

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(K206A/K244A/L301R/V303R/V306R)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX4T3-GST-PolB(L301R/V303R/V306R)

    Plasmid

    #177137

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(L301R/V303R/V306R)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX4T3-GST-PolB(K206A/K244A/T304I)

    Plasmid

    #177134

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPICZ-His6-K2P4.1A-mCherry-StreptagII

    Plasmid

    #158743

    Purpose

    Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-A (KCNK4 Human)
    

    Use

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-264 with N-linked glycosylation sites …

    Promoter

    AOX1

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPICZ-His6-K2P4.1B-mCherry-StreptagII

    Plasmid

    #158744

    Purpose

    Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-B (KCNK4 Human)
    

    Use

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-290 and N-linked glycosylation sites r…

    Promoter

    AOX1

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAD100_hIRG1_4-461_pvp008

    Plasmid

    #124875

    Purpose

    Expresses hCAD Arg273His mutant in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    mutated Arg273 to His, deleted amino acids 1-3 an…

    Promoter

    T7

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLAP-BUBR1-K795A

    Plasmid

    #114061

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

    Insert

    BUBR1-K795A (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance), BUBR1-K795A

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AF10_OM-LZ

    Plasmid

    #111753

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    MLLT10 (MLLT10 Human)
    

    Use

    Tags

    6x His - TEV

    Expression

    Bacterial

    Mutation

    Contains the OM-LZ region of AF10 (residues 720-7…

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGC188

    Plasmid

    #19619

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(attP4-attP3)
    

    Use

    P4-p3 donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRVV601

    Plasmid

    #87632

    Purpose

    RMCE Bxb1 attB-flanked vector.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attB
    

    Use

    Rmce

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGC92

    Plasmid

    #19615

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    FLP
    

    Use

    Carries flp recombinase cdna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGC235

    Plasmid

    #19683

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Plim-7
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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