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pEAHISSIRV418/gp25

Plasmid

#97052

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 418/gp25 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 418/gp25

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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SypHer mt

Plasmid

#48251

Purpose

pH sensor, targeted to mitochondria

Depositor

Nicolas Demaurex

Article

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Tags

2 mitochondrial targeting sequences

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pET28:GFP

Plasmid

#60733

Purpose

pET28 driving expression of GFP, biobrick part Bba_E0040

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGS-21a his6-GST-WTPhi29DNAP

Plasmid

#166863

Purpose

To produce a dual affinity-tagged Phi29 DNA polymerase

Depositor

Paul Blainey, Stephen Quake

Article

Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

Insert

Phi29 Polymerase codon-optimized for overexpression in E. coli

Tags

GST and his6

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9

Plasmid

#128160

Purpose

Ptet-controlled expression of S. pyogenes Cas9

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-postASAP

Plasmid

#178792

Purpose

Expresses voltage sensor targeted to dendrites and spines

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95 and ASAP2f

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJOE6227.1

Plasmid

#135078

Purpose

An integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

up- and downstream regions of upp (upp Pseudomonas putida)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-PERK(LD)-EGFP-HOTag3

Plasmid

#225678

Purpose

Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-UtrCH

Plasmid

#26737

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

GFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261).…

Promoter

CMV IE94

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pInt

Plasmid

#18941

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Mutation

under lacZ promoter

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pLVX-PERK(LD)-EGFP

Plasmid

#225680

Purpose

Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

Depositor

Likun Wang

Article

Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-PRP8

Plasmid

#89966

Purpose

complements prp8 genomic deletion

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

none

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-GFP

Plasmid

#87662

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

eGFP

Use

Retroviral

Promoter

CAG

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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AICSDP-117:POLR2A-mEGFP

Plasmid

#164500

Purpose

Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

Depositor

Allen Institute for Cell Science

Insert

POLR2A Homology Arms with mEGFP-linker (POLR2A Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Feb. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Expression

Bacterial

Mutation

D35-I221

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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OSR1-pCS2+MT

Plasmid

#30491

Depositor

Gerhart Ryffel

Article

Drews et al BMC Dev Biol. 2011 Jan 31;11:5. doi: 10.

Insert

OSR1 (OSR1 Human)

Tags

6xMyc

Expression

Mammalian

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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YIplac211-sGFP-Vrg4

Plasmid

#160904

Purpose

Expresses GFP-Vrg4 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

VRG4

Tags

GFP

Expression

Yeast

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTA-attB

Plasmid

#18937

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attB

Expression

Bacterial

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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PM-ExRai-PKNAR

Plasmid

#226633

Purpose

Plasma membrane-targeted ExRai-PKNAR

Depositor

Ju Chen

Article

Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

Insert

PM-ExRai-PKNAR

Expression

Mammalian

Mutation

Plasma membrane targeting N-terminal 11 amino aci…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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