We narrowed to 7,471 results for: Trac;

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GFP-GLT1b-V5

Plasmid

#162518

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1b (Slc1a2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCa-C1

Plasmid

#112264

Purpose

ITRACKalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase C alpha (PRKCA Bovine)

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-GLT1a-V5

Plasmid

#162517

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pRJPaph-sYFP2

Plasmid

#67033

Purpose

Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
sYFP2

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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Actin Binding Domain Tensionless Control

Plasmid

#215371

Purpose

Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

Depositor

Brenton Hoffman

Article

Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

Actin Binding Domain Tensionless Control

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pWJB9

Plasmid

#126650

Purpose

Expresses AdrA from a modified pLAFR3

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30

Tags

8xHis

Expression

Bacterial

Promoter

PBAD

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-tdTEVDB_C-15A/G4C

Plasmid

#177205

Purpose

Permuted td intron with evolved initiation sequence for the expression of circular mRNA

Depositor

Stephen Fried

Article

Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

Insert

Permuted GFP with evolved initiation sequence for circular mRNA

Tags

6x His

Expression

Bacterial

Mutation

Permuted sfGFP after residue 52 & Changed Cyt…

Promoter

araBAD

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

Plasmid

#166609

Purpose

Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 CA-LRP6-GFP

Plasmid

#29682

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

LDL-receptor related protein 6 (LRP6 Human)

Tags

GFP

Expression

Mammalian

Mutation

extracellular region deleted

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pMRE165

Plasmid

#118513

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAXEF-Rap1-E63(GTP)

Plasmid

#32698

Depositor

Vicki Boussiotis

Article

Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

Insert

RAP1 (RAP1A Human)

Expression

Mammalian

Mutation

E63: Consitutively GTP-bound

Promoter

EF promoter

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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CAG-Cremyc-2A-GFP

Plasmid

#116879

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Article

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCD5-H5_Vietnam_1203/04_sfGFP_ST

Plasmid

#182546

Purpose

Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus

Depositor

Robert de Vries

Article

Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

Insert

H5_Vietnam_1203_04

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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piggybac-EF1-H2AZ-halo

Plasmid

#197907

Purpose

single-particle tracking for H2AZ

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

H2az2 (H2az2 Mouse)

Tags

Halotag

Expression

Mammalian

Promoter

EF1

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMTG06-Amp-FRT

Plasmid

#128275

Purpose

Deg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFP

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Inserts

TetR-LOV-RRRG
EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDendra2-H4

Plasmid

#157815

Purpose

Fluorescent reporter for histone H4 tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H4 (H4c14 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10-Fc-IG

Plasmid

#183722

Purpose

Expresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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