We narrowed to 33,234 results for: eng
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Plasmid#215432PurposeExpression construct for TtA gene, codon optimized for expression in E. coliDepositorInsertTtA
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2_pJEx
Plasmid#213908PurposeBarcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL1-VP1 (LEU2)
Plasmid#182480PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertdeltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.544
Plasmid#184961PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
ExpressionBacterialMutationmurF donor G1359A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Clover4-LUMABS-HA
Plasmid#183486PurposeTo express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.DepositorInsertClover4-LUMABS-HA
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L1-dSpRY
Plasmid#177681PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L2-dSpRY
Plasmid#177682PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L2-dMb2Cas12a
Plasmid#177684PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pb mD3S4-dN
Plasmid#180252PurposeP. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.DepositorInsertD3 of P. berghei HAP2 with N-glycosylation sites mutated
Tags6HisExpressionMammalianMutationN516T, S533N and N539QAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+6A)
Plasmid#171552PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
ExpressionBacterialMutationa six-alanine insertion in the L1 regionAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+3A)
Plasmid#171551PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
ExpressionBacterialMutationa three-alanine insertion in the L1 regionAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD(+6)
Plasmid#171550PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
ExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD
Plasmid#171549PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
ExpressionBacterialAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Xlrbpa
Plasmid#171547PurposeEncoding part of the Xenopus Xlrbpa-2 protein from M120 to L202DepositorInsertXlrbpa (prkra.L Frog)
ExpressionBacterialAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7746_v3
Plasmid#126856Purpose7653 b scaffold from compatibility group 3, made by method II, containing 2 Zn-dependent self-cleaving DNAzyme cassette for lbisection into 2 3780 b scaffoldsDepositorInsertII_CS3_7746_v3
UseSynthetic BiologyAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIs8-4b-SaDAH::G226D
Plasmid#140130PurposeExpresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase G226D variant from Sinomenium acutum
Tags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationchanged Gly226 to Asp226PromoterT7Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-100-1-dendra2
Plasmid#105438Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB HTB_hMSH6_T1219D
Plasmid#135466PurposeExpression human MSH6_T1219D mutant protein using baculovirusDepositorInserthuman MSH6_T1219D (MSH6 Human)
TagsHis-tagExpressionInsectMutationchanged threonine to aspartic acidAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE2-Cerulean
Plasmid#127771PurposeEnhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activityDepositorInsertednrbE2
UseEnhancer reporter constructAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sox3gRNA-T2
Plasmid#127778PurposeChicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)DepositorInsertsox3gRNAT2
UseCRISPRAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only