We narrowed to 6,088 results for: cat.2

1. pGEMHE:mTREK-1(S131C/K271Q)

   Plasmid

   #224750

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C / K271Q

   Promoter

   T7

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMHE:mTREK-1(S131C/G171F)

   Plasmid

   #224749

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(S131C/G171F)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C / G171F

   Promoter

   T7

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEMHE:mTREK-1(S131C/A286F)

   Plasmid

   #224748

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C / A286F

   Promoter

   T7

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV.CBA.YFP.miR-E_shStk16-3

   Plasmid

   #180393

   Purpose

   Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shStk16-3 (Stk16 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ-aC-MSVU-BZ

   Plasmid

   #160426

   Purpose

   Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

   Depositor

   Todd Oakley

   Article

   Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   6x Histidine tag, Yeast alpha secretion factor, c…

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   AOX

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIG-hNFATc2

   Plasmid

   #74050

   Purpose

   retroviral expression plasmid for human NFATc2/C

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human)
   

   Use

   Retroviral

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. RBM10_pLX307

   Plasmid

   #98371

   Purpose

   Lentiviral expression of RBM10

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   RBM10 (RBM10 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Isoform 2

   Promoter

   E1Fa

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. RANLS-DEVD-BNES

   Plasmid

   #50840

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A and ddRFP B
   

   Use

   Tags

   NES sequence LALKLAGLDIGS and triplicated NLS seq…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GANLS-DEVD-BNES

   Plasmid

   #50835

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A and ddRFP B
   

   Use

   Tags

   NES sequence LALKLAGLDIGS and triplicated NLS seq…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MLM3720

    Plasmid

    #49944

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. MLM3762

    Plasmid

    #49947

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CDmut RH-4 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Expression

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

12. CCL2 gRNA (BRDN0001145563)

    Plasmid

    #77866

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CCL2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CCL2 gRNA (CCL2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES2-eGFP:HA-mTREK-1(S131C/A286F)

    Plasmid

    #224775

    Purpose

    Mammalian expression vector for generating mouse TREK-1

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S131C / A286F

    Promoter

    CMV

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEMHE:mTREK-1 (S131A/C159A/C219A)

    Plasmid

    #224753

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131A/C159A/C219A

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEMHE:mTREK-1 (S131C/C159A/C219A)

    Plasmid

    #224752

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C/C159A/C219A

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)

    Plasmid

    #224754

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    C159A/C219A/C365A/C399A

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330_DAZL_cterm2

    Plasmid

    #222919

    Purpose

    Cas9/sgRNA plasmid for targeting DAZL

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, DAZL sgRNA 2 (DAZL Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330_FIGLA_cterm2

    Plasmid

    #222907

    Purpose

    Cas9/sgRNA plasmid for targeting FIGLA

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, FIGLA sgRNA 2 (FIGLA Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118155

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiSAMv2 C2CD4A/B-enh3-guide3

    Plasmid

    #125475

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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