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pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

Plasmid

#213728

Purpose

Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bIV spectrin (repeats 13-15) (Sptbn4 Mouse)

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Puro

Plasmid

#214016

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Zeo

Plasmid

#214017

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pCAGGS

Plasmid

#206054

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Tags

2xHA (internal after Ala526) and GFP

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pcDNA3

Plasmid

#206053

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Tags

2xHA (internal after Ala526) and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-SUMO1

Plasmid

#114388

Purpose

pDONR vector with SUMO1 gene

Depositor

Vincent Dion

Article

Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

Insert

SUMO1 (SUMO1 Human)

Expression

Mammalian

Mutation

Q29H compared with NCBI reference NP_003343.1

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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enz.079-GCN5

Plasmid

#154046

Purpose

Expresses GCN5 in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone acetyltransferase GCN5 (GCN5 Budding Yeast)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available Since

May 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

Plasmid

#148828

Purpose

Mammalian Expression of HsNot9-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-shRNA2res (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pInducer 21-3xFlag-hMEFV-S580X

Plasmid

#195474

Purpose

pInducer21 plasmid containing the human MEFV gene with the S580X mutation (stop codon leading to the truncation of the B30.2 domain of the pyrin protein) with a 3xFlag tag at the N-terminus

Depositor

Thomas Henry

Article

Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

changed S580 to a stop codon

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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mRFP-PA-GFP-RhoAQ63L

Plasmid

#187282

Purpose

Express pmRFP-PAGFP-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

RhoA (RHOA Human)

Tags

mRFP-PAGFP

Expression

Mammalian

Mutation

RhoA Q63L

Promoter

CMV, T7

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTub-alpha-EB3-NeonGreen

Plasmid

#175263

Purpose

labelling of microtubule plus end and F-actin structures

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

EB3 (MAPRE3 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-GFP-AnillinFL

Plasmid

#187272

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6, CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAWK61

Plasmid

#163642

Purpose

rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Nematode, Human)

Use

CRISPR

Tags

2xmKate2 and GFP

Expression

Worm

Promoter

rps-27

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-TK1

Plasmid

#161919

Purpose

Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Thymidine kinase, cytosolic (TK1 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159851

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159843

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Plasmid

#159864

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM322 - array co-injection fragment mosTI neoR

Plasmid

#159832

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI neoR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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K8.1Pr-pGL4.16-OriLyt

Plasmid

#131038

Purpose

Firefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replication

Depositor

Britt Glaunsinger

Article

Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

Insert

KSHV Lytic Origin of replication

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pADSL-2XStrep

Plasmid

#125684

Purpose

expresses human ADSL protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

ADSL (ADSL Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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