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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,084 results for: nls

Showing: 5441 - 5460 of 6084 results

  1. PB-TO-hNGN2

    Plasmid
    #172115
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172115-plasmid-map-sequence-id-448148

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  2. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  3. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  5. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  6. pCLX-UBI-VenusN

    Plasmid
    #27247
    Depositor
    Patrick Salmon
    Insert
    VenusNLS
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    27247-plasmid-map-sequence-id-12850

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  7. PABE-7-NG

    Plasmid
    #138142
    Purpose
    Targeted A to G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-nCas9-NG-3XNLS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138142-plasmid-map-sequence-id-311738

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  8. pPD8_33

    Plasmid
    #1468
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1468-plasmid-map-sequence-id-79

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  9. pPD18_32

    Plasmid
    #1435
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1435-plasmid-map-sequence-id-46

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  10. pPD16_43

    Plasmid
    #1433
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1433-plasmid-map-sequence-id-44

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  11. pPD26_77

    Plasmid
    #1441
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1441-plasmid-map-sequence-id-52

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  12. pPD90_23

    Plasmid
    #1469
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1469-plasmid-map-sequence-id-80

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  13. pDD16_01

    Plasmid
    #1428
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1428-plasmid-map-sequence-id-39

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  14. pPD16_51

    Plasmid
    #1434
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1434-plasmid-map-sequence-id-45

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  15. pPD21_28

    Plasmid
    #1438
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1438-plasmid-map-sequence-id-49

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  16. pPD8_02

    Plasmid
    #1467
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1467-plasmid-map-sequence-id-78

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  17. pPD22_04

    Plasmid
    #1439
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, rf2204, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1439-plasmid-map-sequence-id-50

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  18. pPD22_11

    Plasmid
    #1440
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, rf2211, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1440-plasmid-map-sequence-id-51

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  19. pPD81_34

    Plasmid
    #1457
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    rf8134, IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1457-plasmid-map-sequence-id-68

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  20. pPD18_49

    Plasmid
    #1436
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, myo3N, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1436-plasmid-map-sequence-id-47

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Showing: 5441 - 5460 of 6084 results