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We narrowed to 6,448 results for: nls

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Showing: 5441 - 5460 of 6448 results

  1. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid
    #208952
    Purpose
    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208952-plasmid-map-sequence-id-415741

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  2. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid
    #208956
    Purpose
    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208956-plasmid-map-sequence-id-415745

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  3. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid
    #208946
    Purpose
    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208946-plasmid-map-sequence-id-415680

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  5. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

    Plasmid
    #208958
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208958-plasmid-map-sequence-id-415784

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  14. Protein2PAM_NmeQ981A

    Plasmid
    #250230
    Purpose
    Express Protein2PAM_NmeQ981A nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeQ981A
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 Q981A
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250230-plasmid-map-sequence-id-501768

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  15. Protein2PAM_NmeN1029A

    Plasmid
    #250229
    Purpose
    Express Protein2PAM_NmeN1029A nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN1029A
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 N1029A
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250229-plasmid-map-sequence-id-501766

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  19. pYPQ265K

    Plasmid
    #173997
    Purpose
    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    UNG-rAPOBEC1(R33A)-zCas9(D10A)
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    R33A in rAPOBEC1 and D10A in zCas9
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    173997-plasmid-map-sequence-id-343409

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Showing: 5441 - 5460 of 6448 results