We narrowed to 10,611 results for: t7

1. pRSF-1b-S15-T79C

   Plasmid

   #133048

   Purpose

   Expresses tagless S15-T79C mutant ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   S15 E.coli ribosomal protein (rpsO E.coli)
   

   Expression

   Bacterial

   Mutation

   T79C

   Promoter

   T7

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CcCDP_p15A

   Plasmid

   #179272

   Purpose

   Expresses CcCDP in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Insert

   cellodextrin phosphorylase
   

   Use

   Synthetic Biology

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7 lacO

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

   Plasmid

   #105637

   Purpose

   Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

   Depositor

   Thanos Halazonetis

   Inserts

   Rev7 (Mad2l2 Mouse)
   Rev3l (REV3L Human)
   

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, V132K, C133V, A135K

   Promoter

   T7

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-epELM-5

   Plasmid

   #179391

   Purpose

   Express a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteria

   Depositor

   Shiro Iuchi

   Article

   Iuchi et al Commun Biol. 2021 Dec 15;4(1):1399. doi:

   Insert

   ELMSAN1 (MIDEAS Human)
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7 lac

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCm N-term SMT3 NikJC199U

   Plasmid

   #174210

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, SMT3, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCm C-term SMT3 NikJC199U

   Plasmid

   #174362

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, SMT3, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCm N-term MBP NikJC199U

   Plasmid

   #174364

   Purpose

   Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176575

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NmMetNI-WT

   Plasmid

   #172114

   Purpose

   Expression of MetN and MetI membrane proteins

   Depositor

   Douglas Rees

   Article

   Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

   Inserts

   MetN
   MetI
   

   Tags

   10x His Tag and Enterokinase

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sc NybbR eRF1 (G180A)

    Plasmid

    #175415

    Purpose

    Encodes His-MBP-TEV-ybbR-G180A eRF1

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF1 (SUP45 Budding Yeast)
    

    Tags

    His-MBP-TEV and ybbR

    Expression

    Bacterial

    Mutation

    G180A

    Promoter

    T7

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sc NybbR eRF1 (WT)

    Plasmid

    #175414

    Purpose

    Encodes His-MBP-TEV-ybbR-WT eRF1

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF1 (SUP45 Budding Yeast)
    

    Tags

    His-MBP-TEV and ybbR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis(TEV)-yDrp1-StrepII

    Plasmid

    #174427

    Purpose

    Expresses S. pombe Drp1 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-kcnj20

    Plasmid

    #173150

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj20 (si:ch211-113j13.2 Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-kcnj12b

    Plasmid

    #173142

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj12b (kcnj12b Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-kcnj14

    Plasmid

    #173144

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj14 (kcnj14 Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-kcnj19a

    Plasmid

    #173148

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj19a (kcnj19a Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-kcnj19b

    Plasmid

    #173149

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj19b (kcnj19b Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-kcnj21

    Plasmid

    #173151

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj21 (zgc:162160 Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-kcnj2a

    Plasmid

    #173127

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj2a (kcnj2a Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-kcnj2b

    Plasmid

    #173128

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj2b (kcnj2b Zebrafish)
    

    Use

    Pcr cloning vector

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge