We narrowed to 2,901 results for: aen
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Plasmid#165082PurposegRNA 1 of pair B for Stx5L-specific knockoutDepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCMVAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOD2046-bwmDEG
Plasmid#89367PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)PromoterPmyo-3Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
Tags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-K298M-Y419F-Y530F
Plasmid#140321PurposeExpresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationK298M-Y419F-Y530FPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOD2048-csnDEG
Plasmid#89368PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)PromoterPosm-6Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCC324
Plasmid#110165PurposeminiMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)DepositorInsertERK-KTR-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationR61K change made in ERK-KTR CDSPromoterlin-31 promoter/enhancerAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-hsHEKsite_1_3
Plasmid#205638PurposereRNA guide targeting HEKsite_1_3DepositorAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
hum alpha ENAC promoter a25-a23
Plasmid#83427Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
UseLuciferaseTagsLuciferasePromoteralpha ENACAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pETM6-4F2-mCherry
Plasmid#73421PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.DepositorInsertPromoter 4F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP4F2 (orthogonal T7-lac variant)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only