We narrowed to 7,255 results for: ALP

1. pEF1α -NWM-Node 2

   Plasmid

   #154254

   Purpose

   expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed Node 2 of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1α -NWM-Node 3

   Plasmid

   #154255

   Purpose

   expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed Node 3 of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+-wbp2nlF127P

   Plasmid

   #174507

   Purpose

   expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlF127P
   

   Use

   Xenopus expression

   Tags

   none

   Expression

   Mutation

   changed Phenylalanine at aa127 to Proline

   Promoter

   SP6

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. C453-E21: attB4-FerHp>-attB5

   Plasmid

   #162978

   Purpose

   Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   human Ferritin heavy chain promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. C413-E11: attB4-Grp78p>-attB1r

   Plasmid

   #162970

   Purpose

   Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   human Glucose response protein 78 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. C413-E10: attB4-FerHp>-attB1r

   Plasmid

   #162969

   Purpose

   Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   human Ferritin heavy chain promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Nup54 no FG-mEGFP494(2)

   Plasmid

   #164271

   Purpose

   Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP

   Depositor

   Sanford Simon

   Article

   Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

   Insert

   Nup54 (NUP54 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   deleted the FG region of Nup54

   Promoter

   CMV

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nup54 no FG-mEGFP494(1)

   Plasmid

   #164270

   Purpose

   Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP

   Depositor

   Sanford Simon

   Article

   Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

   Insert

   Nup54 (NUP54 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   deleted the FG region of Nup54

   Promoter

   CMV

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFlox_N-BRM_BSD_FKBPF36V_EYFPF36V

   Plasmid

   #163876

   Purpose

   Acute Depletion of BRM-EYFP

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   Brm (Smarca2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

    Plasmid

    #162811

    Purpose

    Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    BsmBI_(C1A)-Intein_DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Intein(Cys1,Ala)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

    Plasmid

    #162815

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_M
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_M(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

    Plasmid

    #162816

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_9b
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_9b(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

    Plasmid

    #162817

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF6
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF6(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

    Plasmid

    #162818

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF7b
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF7b(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

    Plasmid

    #162819

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF10
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF10(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

    Plasmid

    #162820

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF9c/14
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF9c/14(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

    Plasmid

    #162821

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b_alt
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b_alt(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

    Plasmid

    #162822

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b(CoV1h)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b(CoV1h)(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

    Plasmid

    #162805

    Purpose

    STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI-HA_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIIS18-BufAI-2

    Plasmid

    #128651

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge