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Plasmid#85671PurposeExpression of VCPIP1 with N-terminal GFP tagDepositorInsertVCPIP1 (VCPIP1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-AktAR2
Plasmid#184048PurposeCytosol-targeted ExRai-AktAR2.DepositorInsertCyto-ExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-P2A-BlastR
Plasmid#98147PurposeExpresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsP2A-BlastR fusion with Cas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLV-REPORT(PGK/CMV)-GtwyA
Plasmid#208574PurposeLV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
HSVtk
Neomycin
d2nucEGFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
Plasmid#176587PurposeBacterial expression of His14-MBP-TEV-AmaTnpBDepositorInsertsMBP tag
TnpB
UseTagsHis14 tagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
myristoylated ct-GRK2
Plasmid#178734PurposeN-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)DepositorInsertGRK2 (GRK2 Bovine)
UseLentiviralTagsSrc 1-15ExpressionMutationonly amino acids 548-671PromoterUbiquitinAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1
Plasmid#122083PurposeExpress M.EcoGII fused to LaminB1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-LaminB1
UseRetroviralTagsV5 tag on Nter of LaminB1ExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
SuExp-hPLD4
Plasmid#201244Purposeexpress full length human PLD4 proteinDepositorInsertPLD4 (PLD4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-1- LentiCRISPRv2
Plasmid#107403PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-2- LentiCRISPRv2
Plasmid#107404PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluu
Plasmid#106122PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6A-EGFR ICD (645-1186)
Plasmid#42667DepositorInsertEGFR ICD (645-1186) (EGFR Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-Six1-myc
Plasmid#138530PurposeInducible expression of Six1 with a myc tagDepositorInsertSix1 (Six1 Mouse)
UseTags3x myc tagExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPPEDs
Plasmid#162469Purposeprotoplast test vector of pPPEDDepositorInsertsCas9H840A
attR gateway destination cassette
UsePrime editing destination vector for transient ex…TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlaninePromoterAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-Neuromodulin-IRES-Puromycin
Plasmid#134666PurposeExpresses EGFP-tagged plasma membrane markerDepositorInsertNeuromodulin (GAP43 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only