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Showing: 10961 - 11000 of 17820 results

  1. pKB233.2

    Plasmid
    #179999
    Purpose
    Encodes PmgtA-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Insert
    mNeonGreen
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179999-plasmid-map-sequence-id-360289

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  2. pKB233.1

    Plasmid
    #179998
    Purpose
    Encodes Pmig-14-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Insert
    mNeonGreen
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179998-plasmid-map-sequence-id-360277

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  3. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Available Since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

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  4. pInt_attP1_LCS_kanR

    Plasmid
    #205300
    Purpose
    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205300-plasmid-map-sequence-id-407004

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  6. XLone-Puro eGFP

    Plasmid
    #140027
    Purpose
    Tunable and temporal expression control of nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140027-plasmid-map-sequence-id-275840

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  7. pT7TS-rich

    Plasmid
    #99050
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Unspecified; Xenopus oocyte system
    Promoter
    pT7
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    99050-plasmid-map-sequence-id-191760

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  13. pCI_FLAG-GFP(Y39TAG)-6His

    Plasmid
    #223507
    Purpose
    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39
    Depositor
    Edward Lemke
    Insert
    GFP(Y39TAG)
    Tags
    6xHis and FLAG
    Expression
    Mammalian
    Mutation
    Y39TAG
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223507-plasmid-map-sequence-id-447435

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  14. pCDNA_Lifeact-GFP_NLS-mCherry

    Plasmid
    #69058
    Purpose
    Dual Fluorescent Reporter for F-actin and Nuclei
    Depositor
    Olivier Pertz
    Insert
    LifeAct-GFP_IRES_NLS-mCherry
    Tags
    Lifeact-GFP, NLS-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69058-plasmid-map-sequence-id-123543

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  15. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    6xhis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  17. mGeos-M-C1

    Plasmid
    #54815
    Purpose
    Localization: C1 Cloning Vector, Excitation: 503, Emission: 514, reversibly switches on/off with UV. (Originally reported as Excitation: 503 / 569, Emission: 514 / 581)
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mGeos-M
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  20. pGL3_FAM153CP_SOX9rep

    Plasmid
    #232400
    Purpose
    Enhancer near FAM153CP, buffering element replaced with SOX9 site
    Depositor
    Joanna Wysocka
    Insert
    FAM153CP (FAM153CP Human)
    Use
    Luciferase
    Mutation
    Enhancer near FAM153CP, buffering element replace…
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232400-plasmid-map-sequence-id-462845

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  21. pGL3-alphaMHC 3300bp

    Plasmid
    #51934
    Purpose
    luciferase reporter for rat alpha-MHC promoter
    Depositor
    Jeffery Molkentin
    Insert
    alpha MHC (Myh6 Rat)
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    -2930 to +410 promoter region
    Promoter
    alpha MHC
    Available Since
    May 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. pET28-3'TwStrep

    Plasmid
    #208053
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208053-plasmid-map-sequence-id-415923

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  25. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208620-plasmid-map-sequence-id-415919

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  26. pKT-mPDGFb-IRES-Katushka

    Plasmid
    #133082
    Purpose
    Expresses mouse PDGFb and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    PDGFb (Pdgfb Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133082-plasmid-map-sequence-id-261113

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  27. mEos4b-Nup50-N-10

    Plasmid
    #57516
    Purpose
    Localization: Nuclear Pore, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    Nup50 (NUP50 Human)
    Tags
    mEos4b
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pAIP4_4WDC

    Plasmid
    #234153
    Purpose
    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4WDC
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234153-plasmid-map-sequence-id-464119

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  29. pHD-w+

    Plasmid
    #80927
    Purpose
    Vector for generating dsDNA donors for HDR. Empty backbone containing a StuI restriction site and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Insert
    GMR-white
    Use
    High copy, amp resistance
    Promoter
    GMR::hsp70
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80927-plasmid-map-sequence-id-152786

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  32. mGeos-M-N1

    Plasmid
    #54753
    Purpose
    Localization: N1 Cloning Vector, Excitation: 503, Emission: 514, reversibly switches on/off with UV. (Originally reported as Excitation: 503 / 569, Emission: 514 / 581)
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mGeos-M
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  37. EGFP-hPMCA4bct125

    Plasmid
    #47594
    Depositor
    Emanuel Strehler
    Insert
    PMCA4bct125 (ATP2B4 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    125 C terminal AA deleted
    Promoter
    CMV
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. Pf-PPWD1

    Plasmid
    #25569
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pf-PPWD1 (PFE0505w Plasmodium falciparum)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 10961 - 11000 of 17820 results