We narrowed to 13,953 results for: LEA

Showing: 5501 - 5520 of 13953 results

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pET21-pelB-LaG-10

Plasmid

#172743

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-10

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-6

Plasmid

#172741

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-pelB-LaG-42

Plasmid

#172758

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-42

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry_eDHFR_SIM#1

Plasmid

#164646

Purpose

tag SIM with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Publication

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIM

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DinoIII-arrO-N

Plasmid

#128143

Purpose

Dinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marina

Depositor

Senjie Lin

Publication

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

Rifampin ADP-ribosylating transferase + potential promoter

Expression

Bacterial

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_RcCHAD

Plasmid

#127782

Purpose

Expresses RcCHAD in E. coli cells

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

RcCHAD

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWY

Plasmid

#112901

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Publication

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDFNKI-his-3C-LIC-strep

Plasmid

#108705

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Publication

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

His-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LEXY TR (without KRAB domain) (pDN162)

Plasmid

#72661

Purpose

LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repression

Depositor

Barbara Di Ventura, Roland Eils

Publication

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LexATF-T2A-NLS-LexA-mCherry-LEXY

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGoldyTALEN-VIM-TAL-2

Plasmid

#62852

Purpose

TALEN mediated gene editing at human VIM locus

Depositor

Gaudenz Danuser

Publication

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Expression

Mammalian

Promoter

miniCAGG

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGoldyTALEN-VIM-TAL-1

Plasmid

#62855

Purpose

TALEN mediated gene editing at human VIM locus

Depositor

Gaudenz Danuser

Publication

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

TALEN array

Use

TALEN

Expression

Mammalian

Promoter

miniCAGG

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pET23a_mod_RBPMS2_FL

Plasmid

#59394

Purpose

Plasmid for bacterial overexpression of RBPMS2

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Tags

6xHis, FLAG, HA, and T7

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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NET37 pEGFP-N2 (607)

Plasmid

#61990

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NET37 (KIAA1161 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
NET39 pEGFP-C1 (1045)

Plasmid

#62024

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NET39 (PPAPDC3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-831-1281

Plasmid

#40962

Depositor

Elizabeth Luna

Publication

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830 and 1282-1792

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
M47 MBP-TEV-Chibby

Plasmid

#17195

Depositor

Randall Moon

Publication

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Tags

MBP

Expression

Bacterial

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
MUM1

Plasmid

#28273

Depositor

Cheryl Arrowsmith

Insert

MUM1 (MUM1 Human)

Tags

His with TEV cleavage

Expression

Bacterial

Mutation

Contains PWWP Domain (residues 405-538).

Available since

Feb. 10, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pEMS1473

Plasmid

#29234

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple3 (ADORA2A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Jan. 9, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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