We narrowed to 13,747 results for: Cre

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pWZL Neo Myr Flag CKB

Plasmid

#20466

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKB (CKB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

Plasmid

#133339

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Publication

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF3_P2A_Hygro_Barcode

Plasmid

#120490

Purpose

Barcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF3 (KLF3 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MESP1_P2A_Hygro_Barcode

Plasmid

#120459

Purpose

Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MESP1 (MESP1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1L

Plasmid

#33242

Depositor

Huda Zoghbi

Publication

Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

Insert

ATXN1L (ATXN1L Human)

Tags

Flag

Expression

Mammalian

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAC-EsaR-D91G

Plasmid

#47646

Purpose

pAC-EsaR with a a272g mutation in esaR

Depositor

Cynthia Collins

Publication

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-D91G

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Aspartic acid 91 to Glycine

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

Plasmid

#216164

Purpose

Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Publication

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

Gaussia princeps luciferase (with double mutation to improve stability)

Expression

Mammalian

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-TetO-PM

Plasmid

#197387

Purpose

To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

Depositor

Qiao Zhou

Publication

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral; Teto

Expression

Mammalian

Promoter

TRE

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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iBE4max YE1 NG CLYBL

Plasmid

#174570

Purpose

doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

Depositor

Mathew Garnett

Publication

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

BE3

Expression

Mammalian

Promoter

tet ON

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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m.Setd7

Plasmid

#87131

Purpose

mammalian expression of Setd7

Depositor

Francesca Spagnoli

Publication

Kofent et al Development. 2016 Oct 1;143(19):3573-358

Insert

Setd7 (Setd7 Mouse)

Tags

6x-Myc

Expression

Mammalian

Promoter

simian CMV IE94

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET29b-Ava3858-3855

Plasmid

#45746

Depositor

Emily Balskus, Christopher T Walsh

Publication

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3855

Expression

Bacterial

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pAG306-GPD-ccdB chr I

Plasmid

#41894

Depositor

Dan Gottschling

Publication

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Expression

Yeast

Promoter

GPD

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_nV5-AMOTL1

Plasmid

#172998

Purpose

constitutive expression of V5-tagged AMOTL1

Depositor

Michael Wehr

Publication

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOTL1 (AMOTL1 Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP CMV GFP1-9

Plasmid

#130271

Purpose

Lentiviral expression vector for stable expression of GFP1-9

Depositor

Stéphanie Cabantous

Publication

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

GFP 1-9 OPT

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

CMV

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-CalfluxCTN

Plasmid

#190068

Purpose

Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

Depositor

Carl Johnson

Publication

Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

Insert

CalfluxCTN

Expression

Mammalian

Promoter

CAG promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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hMTP

Plasmid

#124281

Purpose

Expresses human MTP in mammalian cells.

Depositor

Mahmood Hussain

Publication

Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

Insert

MTTP (MTTP Human)

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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OPRM1-DuET

Plasmid

#213361

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPRM1 (OPRM1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pλPR cI-Elysis

Plasmid

#176891

Purpose

Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

Depositor

James Inglese

Publication

Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

Insert

Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system

Expression

Bacterial

Promoter

T7

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1adCas9VP64T2ABsd-W

Plasmid

#112919

Purpose

Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

dCas9VP64-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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