We narrowed to 7,530 results for: Ski;
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Plasmid#159749PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRExpressionPlantAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
FokI(inactive)-mCherry-LacI-NLS
Plasmid#215100PurposeExpresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.DepositorInsertFokI(inactive)-mCherry-LacI-NLS
ExpressionMammalianMutationFokI(inactive) mutant D69A.PromoterCMVAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CDKN1A (p21) targeting gRNA
Plasmid#215319PurposeExpresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.DepositorInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX CblB-SH2
Plasmid#46413DepositorInsertCblB (CBLB Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 42-359PromoterTacAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-Venus-iGluSnFR.A184S
Plasmid#106195PurposeTight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184S
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184SPromoterGFAPAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.S72A
Plasmid#106203PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterCAGAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-BAP1-C91S-HA
Plasmid#154021PurposeMammalian Expression of HA-tagged p.C91S Mutant BAP1DepositorInsertBAP1 (BAP1 Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationp.C91S (Missense Mutation, Catalytically Inactive)Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-mScarlet-I-Cdt1
Plasmid#191100PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertmScarlet-I-Cdt1 (30-120)
UseAAV and Synthetic BiologyTagsmScarlet-I fused to residues 30-120 of human Cdt1…ExpressionMammalianMutationOptimized to human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-BRCA1 3'UTR
Plasmid#29477DepositorInsertBRCA1 3'UTR (BRCA1 Human)
ExpressionMammalianAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt
Plasmid#157702Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoterDepositorInsertNsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
TagsHis8-FlagExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
human rSHIP2 (18-893)
Plasmid#128568PurposeExpression of human SHIP2 in bacteriaDepositorInsertSHIP2 (INPPL1 Human)
TagsHis Xpress (N-terminal)ExpressionBacterialMutationSHIP2 coding sequence from amino acid 18 (A) till…Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Btk-SH2
Plasmid#46412DepositorInsertBtk (BTK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 271-385PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-SAE1_sh1
Plasmid#168984PurposeExpresses RFP cDNA and SAE1 shRNA 1 in mammalian cellsDepositorAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-HNRNPD_sgRNA1
Plasmid#201598PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertHNRNPD (HNRNPD Human)
UseCRISPR and LentiviralAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AT5G62710 X045_pECIA2
Plasmid#115130PurposeBait vector AT5G62710 X045_pECIA2 should be used with prey vector AT5G62710 X045_pECIA14.DepositorInsertAT5G62710 (AT5G62710 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
BE3-NLS-NLS-P2A-BLAST
Plasmid#135348PurposeExpression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertBE3-2XNLS
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AT1G35710 X075_pECIA2
Plasmid#115098PurposeBait vector AT1G35710 X075_pECIA2 should be used with prey vector AT1G35710 X075_pECIA14.DepositorInsertAT1G35710 (AT1G35710 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-hAGT-His
Plasmid#167276PurposeOverexpression of the human o_-alkylguanine-dna alkyltransferase in E. coliDepositorAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AT1G56140 X032_pECIA2
Plasmid#115062PurposeBait vector AT1G56140 X032_pECIA2 should be used with prey vector AT1G56140 X032_pECIA14.DepositorInsertAT1G56140 (AT1G56140 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G06840 X062_pECIA2
Plasmid#115052PurposeBait vector AT1G06840 X062_pECIA2 should be used with prey vector AT1G06840 X062_pECIA14.DepositorInsertAT1G06840 (AT1G06840 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only