We narrowed to 5,941 results for: pCas

Showing: 5521 - 5540 of 5941 results

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myc-hRAD18 dSAP

Plasmid

#68830

Purpose

Expresses c-myc-tagged human RAD18 deleting SAP domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
SIN-PGK-Cas9-V5-WPRE

Plasmid

#87876

Purpose

To produce lentiviral vector for Cas9 editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

spCas9

Use

Lentiviral

Tags

V5

Mutation

Human codon-optimized

Promoter

mouse PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 dC2

Plasmid

#68831

Purpose

Expresses c-myc-tagged human RAD18 deleting Polymerase eta binding domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pX459_HR_Prnp_3

Plasmid

#78623

Purpose

pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pX330 NC-FKBP-Cas9

Plasmid

#162725

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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FL MALT1 Catalytic Mutant in pET29b-Strep

Plasmid

#48969

Purpose

Expresses Full length catalytic mutant MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Tags

His and Strep

Expression

Bacterial

Mutation

Catalytic Mutant C464A

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
CAG-RORb2

Plasmid

#73999

Purpose

Overexpression of RORb2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

RORb2 ORF (Rorb Mouse)

Tags

none

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

Plasmid

#160616

Purpose

TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 d6BD

Plasmid

#68832

Purpose

Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)

Tags

c-myc

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
CAG-RORb2-IRES-GFPd2

Plasmid

#73998

Purpose

Plasmid expressing RORb2-IRES-GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

RORb2 ORF (Rorb Mouse)

Tags

none

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pX330 L-FKBP-Cas9

Plasmid

#162728

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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lentiGuideFB-Puro-B

Plasmid

#192507

Purpose

For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE

Use

Lentiviral

Promoter

bU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLCHKO_HPRT1_exon3_2_Lb

Plasmid

#155054

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJT131_GalL_cCDA1-NGBE3

Plasmid

#145095

Purpose

Expressing base editor cCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET21 Catalytic Domain of MALT1 Catalytic Mutant

Plasmid

#48971

Purpose

Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566; Catalytic Mutant C464A

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
SIN-CMV-Ca9-WPRE

Plasmid

#87874

Purpose

Transfer plasmid for the production of lentiviral vectors

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

spCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC2360 - pAAV rActin EGFP donor

Plasmid

#228444

Purpose

An adeno-associated viral vector to express a SpCas9 guide RNA targeting rat beta actin also carrying an HDR template for insertion of mEGFP to the break site.

Depositor

Christopher Richie

Article

Ribeiro Gomes et al Cell Rep. 2025 Dec 21;45(1):116756. doi:

Insert

mEGFP

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pSA143 Cas9-BFP with core cHS4 insulators

Plasmid

#249153

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-TagBFP with core cHS4 insulators

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-TAF1-A

Plasmid

#247346

Purpose

Expresses SpCas9 and a sgRNA targeting the human TAF1-A loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1 (TAF1 Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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