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Plasmid#171075PurposeExpresses Gal4-vp16 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
UseTagsHis and VP16 AD (413-490)ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-GFP-IRES-mCherry
Plasmid#226098PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTLR3-pcDNA3
Plasmid#32712DepositorInsertTLR3 (TLR3 Human)
UseTagsFLAG and PreprototrypsinExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-polg guide
Plasmid#107598PurposeAn entry vector with U6a promoter driving polg guide RNA expressionDepositorInsertpolg guide RNA sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCD-Ugi22
Plasmid#207004PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)DepositorInsertUtricularia gibba insulator 22
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCD-Ugi21
Plasmid#207005PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)DepositorInsertUtricularia gibba insulator 21
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTALdNC-AAVS1_T1
Plasmid#80495PurposeAAVS1 TALEN expression vector (Left)DepositorInsertAAVS1 TALEN (Left)
UseGateway entryTagsExpressionMutationPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTALdNC-AAVS1_T2
Plasmid#80496PurposeAAVS1 TALEN expression vector (Right)DepositorInsertAAVS1 TALEN (Right)
UseGateway entryTagsExpressionMutationPromoternoneAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP17_up1691, P20_992, and iP16_3622Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUD-MSP1D1
Plasmid#161608PurposeBacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans and tyrosines w…PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2R3z-BASI-ccdB-BSAI
Plasmid#118389PurposeEntry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertccdB
UseUnspecifiedTagsExpressionMutationSee Depositor Comments SectionPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGR
Plasmid#46002PurposeReporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinoseDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationSRDX (X3) repressor fusion; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-MCS
Plasmid#75174PurposeMultiple cloning site for insertion of gene of interest for C-terminal fusions.DepositorInsertMultiple Cloning Site
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-tRNA2.0
Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-TagRFP-t35s
Plasmid#118386PurposeEntry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p-TagRFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-RZ-Cas12j2
Plasmid#189784PurposeGolden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD-MSP1D1
Plasmid#161607PurposeBacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formationDepositorInsertD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans were replaced b…PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TurboID-mNeongreen-3xFLAG fusion pos3
Plasmid#172864PurposeC. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion proteinDepositorInsertTurboID
UseUnspecifiedTagsFLAG, mNeongreen, and mycExpressionMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only