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YEE-NG-CBE

Plasmid

#227418

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Publication

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L858R

Plasmid

#116276

Purpose

Lentiviral expression of EGFR L858R

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

L858R

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Publication

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLenti/TO/EYFP-P2A-IDH1(HA)

Plasmid

#122482

Purpose

A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA

Depositor

Eric Huang

Publication

Tiburcio et al Acta Neuropathol. 2018 Feb;135(2):285-29

Insert

isocitrate dehydrogenase 1 (IDH1 Human)

Use

Lentiviral

Tags

HA and P2A

Expression

Mammalian

Promoter

CMV/TO

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Publication

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAG426GPD-ccdB-HA

Plasmid

#14252

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

3HA

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAG426GAL-EGFP-ccdB

Plasmid

#14323

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG413GPD-ccdB

Plasmid

#14142

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-EXO1b

Plasmid

#111621

Purpose

Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

Depositor

Marco Muzi-Falconi

Publication

Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

Insert

Exonuclease 1b (EXO1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL3 basic E2

Plasmid

#48747

Purpose

Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Publication

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region containing E-box2 (-112 to +98)

Use

Luciferase

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Annexin V-sfGFP

Plasmid

#257992

Purpose

Bacterial expression of fluorescent Annexin V

Depositor

Michael Marty

Publication

Cleveland et al ACS Chem Biol. (2026). doi: https://doi.

Insert

ANXA5 (ANXA5 Human)

Tags

sfGFP

Expression

Bacterial

Promoter

T7 Promoter

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wtΔKDEL YD/FL

Plasmid

#214805

Purpose

Mammalian expression of human CALR wtΔKDEL YD/FL

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR wtΔKDEL YD/FL (CALR Human)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR del52-His

Plasmid

#214795

Purpose

Baculovirus expression of human CALR del52-His

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52-His (CALR Human)

Tags

His

Expression

Insect

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR del52-linker-Venus

Plasmid

#214788

Purpose

Mammalian expression of human CALR del52-linker-Venus

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR del52-linker-Venus (CALR Human)

Use

Retroviral

Promoter

MSCV

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV/CALR ins5-linker-Venus

Plasmid

#214789

Purpose

Mammalian expression of human CALR ins5 -linker-Venus

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR ins5-linker-Venus (CALR Human)

Use

Retroviral

Promoter

MSCV

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wtΔKDEL-FLAG-KDEL

Plasmid

#214792

Purpose

Mammalian expression of human CALR wtΔKDEL-FLAG-KDEL

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALRwtΔKDEL-FLAG-KDEL (CALR Human)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Flag-SOD1-G86R

Plasmid

#235663

Purpose

Express human SOD1-G86R in mammalian cells

Depositor

Chen Seng Ng

Publication

Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

Insert

SOD1-G86R (SOD1 Human)

Tags

Flag-tag at N-terminal

Expression

Mammalian

Mutation

changed Glycine 86 to arginine

Promoter

CMV promoter

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Flag-SOD1-D91A

Plasmid

#235664

Purpose

Express human SOD1-D90A in mammalian cells

Depositor

Chen Seng Ng

Publication

Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

Insert

SOD1-D91A (SOD1 Human)

Tags

Flag-tag at N-terminal

Expression

Mammalian

Mutation

changed Aspartic acid 91 to alanine

Promoter

CMV promoter

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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AID-C12*-n'Cas9-BE4max

Plasmid

#216730

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

Depositor

Rahul Kohli

Publication

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-n'Cas9-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

Plasmid

#212539

Purpose

Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Publication

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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