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Showing: 5541 - 5560 of 62659 results

  1. SpCas9-NG-YE1-BE4max

    Plasmid
    #198535
    Purpose
    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198535-plasmid-map-sequence-id-388892

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  3. plRL19

    Plasmid
    #163634
    Purpose
    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).
    Depositor
    Jeremy Rock
    Article
    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00
    Insert
    L5 Integrase
    Expression
    Bacterial
    Promoter
    Mycobacterial Optimized Promoter (MOP) and Tet-On
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163634-plasmid-map-sequence-id-339549

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  5. pBS31-cCARLIN

    Plasmid
    #160928
    Purpose
    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)
    Depositor
    Fernando Camargo
    Article
    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.
    Insert
    CARLIN gRNAs and CAG-GFP-CARLIN array
    Use
    Mouse Targeting
    Expression
    Mammalian
    Promoter
    U6 promoter for gRNAs; CAG promoter for GFP
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160928-plasmid-map-sequence-id-421629

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  6. LiOn-CAG∞IRFP

    Plasmid
    #154017
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    IRFP670
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154017-plasmid-map-sequence-id-308109

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  9. pMM643

    Plasmid
    #112533
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Expression
    Bacterial
    Mutation
    A17P
    Promoter
    J23107, PL(HrtO), and ProD
    Available Since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112533-plasmid-map-sequence-id-216001

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  13. iOn-CAG∞RFP

    Plasmid
    #154014
    Purpose
    Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    mRFP1
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154014-plasmid-map-sequence-id-308093

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  17. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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Showing: 5541 - 5560 of 62659 results