We narrowed to 18,226 results for: gateway

1. pLgw NKX2-5Y191C-V5-EcoDam

   Plasmid

   #98599

   Purpose

   Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 (Nkx2-5 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Mutation

   Y191C point mutant

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. KHBD00026

   Plasmid

   #34188

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG8333 (E(spl)mgamma-HLH Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLENTI_TetOn_Hu_BIRC5(WT)

   Plasmid

   #136348

   Purpose

   Lentiviral inducible expression (TetOn) of human BIRC5

   Depositor

   Guillermo de Cárcer

   Insert

   BIRC5 (BIRC5 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

   Plasmid

   #175573

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST51-LRRK2-G2019S

   Plasmid

   #29401

   Depositor

   Mark Cookson

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Destination vector

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Gly2019Ser

   Available Since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

   Plasmid

   #175574

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST51-LRRK2-R1398Q

   Plasmid

   #29398

   Depositor

   Mark Cookson

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Destination vector

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Arg1398Gln

   Available Since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pHAGE mCherry-Rab11A

   Plasmid

   #228035

   Purpose

   Mammalian lentiviral expression of mCherry-labeled Rab11.

   Depositor

   Wade Harper

   Insert

   Rab11A (RAB11A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LZRS-Rfa

   Plasmid

   #31601

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Type

   Empty backbone

   Use

   Retroviral

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. IDG_CACNG8_OE_1

    Plasmid

    #161698

    Purpose

    Overexpresses CACNG8

    Depositor

    Mike McManus

    Insert

    CACNG8 (CACNG8 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Dec. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Phage-PGK-GFP-T(WT)

    Plasmid

    #181736

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid

    #169389

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP16
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCLX-R4-DEST-R2

    Plasmid

    #45956

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Methods Mol Biol. 2011;737:183-209. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307-TMPRSS4-puro

    Plasmid

    #158472

    Purpose

    Constitutive lentiviral expression of human TMPRSS4.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    TMPRSS4 (TMPRSS4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG426-GAL-UBQLN2

    Plasmid

    #181708

    Purpose

    Galactose inducible expression of UBQLN2

    Depositor

    Alejandro Chavez

    Insert

    UBQLN2 (UBQLN2 Human)
    

    Expression

    Yeast

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCWXPG-UBI-Glis3M412

    Plasmid

    #114301

    Purpose

    Ubiquitous expression of a constitutively active form of the Glis3 transcription factor

    Depositor

    Patrick Salmon

    Insert

    Glis3 (Glis3 Mouse)
    

    Use

    Lentiviral

    Mutation

    N-terminal deletion up to Methionine 412

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER20-hMYF5-3xHA (#732)

    Plasmid

    #78332

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    MYF5 (MYF5 Human)
    

    Use

    Lentiviral

    Tags

    3x HA tag

    Promoter

    TRE2

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pENTR223 TMPRSS2, closed

    Plasmid

    #149721

    Purpose

    for Gateway cloning of TMPRSS2 into destination vector

    Depositor

    Anne-Claude Gingras

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Entry vector

    Mutation

    wt, without start codon

    Available Since

    June 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA-SUN1 mApple

    Plasmid

    #187676

    Purpose

    In vivo visualization of SUN1

    Depositor

    Mary Beckerle

    Insert

    SUN1 (Sun1 Mouse)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-SUN1 EGFP

    Plasmid

    #187675

    Purpose

    In vivo visualization of SUN1

    Depositor

    Mary Beckerle

    Insert

    SUN1 (Sun1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPtGG-1

    Plasmid

    #89990

    Purpose

    pBR322-based entry vector for Gateway cloning

    Depositor

    Philip Weyman

    Article

    Bielinski et al PeerJ Preprints. 2017 5:e2907v1

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Available Since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid

    #169384

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP10
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. IDG_CACNG7_OE_1

    Plasmid

    #161651

    Purpose

    Overexpresses CACNG7

    Depositor

    Mike McManus

    Insert

    CACNG7 (CACNG7 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti4/V5-DEST-P85(KS459del)

    Plasmid

    #40222

    Depositor

    Lynda Chin

    Insert

    P85(KS459delN) (PIK3R1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    amino acids 459-460 replaced with asparagine

    Promoter

    CMV

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid

    #169391

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF3b
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAM-35s-CBL2-YFPc-1

    Plasmid

    #102394

    Purpose

    split YFP. Plant expression of CBL2-YFPc-1

    Depositor

    Silke Robatzek

    Insert

    CBL2 (CBL2 Mustard Weed)
    

    Tags

    YFPc

    Expression

    Plant

    Promoter

    35S

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pENTR-HLA-B15-His

    Plasmid

    #104837

    Purpose

    HLA-B150101 cloned as a His tag

    Depositor

    Sébastien Walchli

    Insert

    major histocompatibility complex, class I, B (HLA-B Human)
    

    Use

    Entry vector for gateway system

    Tags

    his

    Mutation

    2 differences compared to original B15: L2->R …

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pINDUCER20-FLAG-hMYF5 (#801)

    Plasmid

    #78334

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    MYF5 (MYF5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    TRE2

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pLV-SspB-HaloTag-TRIO-DHPH1

    Plasmid

    #176126

    Purpose

    Heterodimerization with iLID, Rac1 and RhoG activation

    Depositor

    Dorus Gadella

    Insert

    TRIO (TRIO Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDEST51-LRRK2-I1371V

    Plasmid

    #29399

    Depositor

    Mark Cookson

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Ile1371Val

    Available Since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only