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pMCh-664

Plasmid

#92252

Purpose

Plasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

EF1A

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-658

Plasmid

#92257

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif

Depositor

Marina Chekulaeva, Witold Filipowicz

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

NHA

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 2

Plasmid

#99317

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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RSV/mGATA3 (antisense)

Plasmid

#83817

Purpose

contains antisense mouse GATA3

Depositor

Douglas Engel

Article

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

GATA3-antisense strand (Gata3 Mouse)

Expression

Mammalian

Promoter

RSV

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pPD95.75 RSF-1

Plasmid

#66863

Purpose

Generate transgenic C..elegans expressing GFP-fused RSF-1

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

RSF-1 (rsf-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_KDR_111del

Plasmid

#65659

Purpose

FLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced stop codon such as to produce deletion…

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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Gal4pBS13-Pleum

Plasmid

#60332

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

May 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCVD403

Plasmid

#63740

Purpose

Contains the LT gene probe (HincII fragment) cloned on BamHI linkers into pACYC184

Depositor

James Kaper

Article

Gicquelais et al J Clin Microbiol. 1990 Nov;28(11):2485-9

Insert

LT gene probe

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

Plasmid

#163027

Purpose

FLiCRE TF with fast kinetics for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

CD4-MKII-f-hLOV1-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HSF1Δ∼PRD

Plasmid

#71725

Purpose

plasmid expressing mic-his tag phosphorylation-deficient hHSF1

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

phosphorylation-deficient HSF1 (HSF1 Human)

Tags

mac 6xHIS

Expression

Mammalian

Mutation

15 phosphorylation sites in RD mutated to Alanine

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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Blue opsin mcherry ---- pcDNA3.1

Plasmid

#62932

Purpose

Blue opsin tagged with mcherry on the C terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

blue opsin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pWZL Sox2 R74P

Plasmid

#26355

Depositor

Matthew Meyerson

Article

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

Sox2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R74P

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR (DRR728)

Plasmid

#248232

Purpose

Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attP target sites

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605)

Plasmid

#248211

Purpose

bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Stab-exin21-Bxb1(A315R)-Stab

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

Bxb1(A315R)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-EYFP

Plasmid

#231926

Purpose

Cre-on/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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TdLanYFP-AURKA-Aquamarine

Plasmid

#228562

Purpose

Encodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.

Depositor

Giulia Bertolin

Article

Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

Insert

AURKA (AURKA, AURKA Human)

Tags

Aquamarine and TdLanYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

Plasmid

#178518

Purpose

For making Rabies virus SAD B19 strain L cell line with hyPBase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

B19L-IRES-mCherry

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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