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pAAV.GFAP.(cyto).iGlucoSnFR2.HaloTag

Plasmid

#244085

Purpose

Cytosolic expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PB-U6-CNCB_sgSOX2

Plasmid

#242175

Purpose

A piggybac-based vector containing mouse U6 promoter-driven SOX2 sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

SOX2 (SOX2 Human)

Use

Piggybac

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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PB-U6-CNCB_sgPOU5F1

Plasmid

#242174

Purpose

A piggybac-based vector containing mouse U6 promoter-driven POU5F1sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

POU5F1 (POU5F1 Human)

Use

Piggybac

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pYO843

Plasmid

#242487

Purpose

Expression of ScAtg38 Δ(210 to 226)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 210 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO835

Plasmid

#242484

Purpose

Expression of ScAtg38 Δ(123 to 181)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 181

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO842

Plasmid

#242486

Purpose

Expression of ScAtg38 Δ(123 to 209)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO817

Plasmid

#242483

Purpose

Expression of ScAtg38 Δ(182 to 226)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO836

Plasmid

#242485

Purpose

Expression of ScAtg38 Δ(182 to 209)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 182 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLD1112 TRE-mCherry-SKL-22Xm6A MS2

Plasmid

#235128

Purpose

mCherry reporter plasmid for live cell visualization of RNA m6A modification

Depositor

Qin Peng

Article

Li et al Sci Adv. 2024 Nov 15;10(46):eadp5689. do

Insert

TRE-mCherry-SKL-22Xm6A MS2

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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yTREX-ApR

Plasmid

#177307

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW21K_1Ti1

Plasmid

#177291

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

eYFP

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW27K_7Ti1

Plasmid

#177294

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZ

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK56K_3G7

Plasmid

#177301

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK58K_6G7

Plasmid

#177302

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTSK65K_8G7

Plasmid

#177303

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and GUS

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

uidA

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW07K_0G5

Plasmid

#177279

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PaacC1-aacC1

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW08K_0C5

Plasmid

#177280

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Pcat-cat

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW11K_0S5

Plasmid

#177282

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with SmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PaadA-aadA

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW14K_7G5

Plasmid

#177283

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and LacZ

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

lacZ

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW15K_2G5

Plasmid

#177284

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mTagBFP2

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mTagBFP2

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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