We narrowed to 8,725 results for: pcDNA3

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nERb446-gs10-NLS-VPR

Plasmid

#188263

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nERb446 (ESR1 Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Epac-S-H90

Plasmid

#170340

Purpose

CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

Insert

CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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cpGFP-Adhiron_flex

Plasmid

#165044

Purpose

Flexible linker control for POLArISact expression in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

cpGFP-Adhiron (connected with a flexible linker)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNAIIIB HA JNK

Plasmid

#61630

Purpose

mammalian expression of JNK

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

JNK1 beta1 isoform (MAPK8 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
LIC-Z

Plasmid

#153543

Purpose

perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Tags

mCherry

Expression

Mammalian

Mutation

NA

Promoter

CMV

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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Human Inositol 1,4,5-Trisphosphate 3-Kinase B

Plasmid

#122589

Purpose

Expression of human inositol 1,4,5-trisphosphate 3-kinase B

Depositor

Christophe Erneux

Article

Dewaste et al Biochem Biophys Res Commun. 2002 Feb 22;

Insert

inositol 1,4,5-trisphosphate 3-kinase B (ITPKB Human)

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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Human HMG-CoA lyase like 1 (HMGCLL1)

Plasmid

#52434

Purpose

mammalian expression of human HMG-CoA lyase like 1 (HMGCLL1)

Depositor

Henry Miziorko

Article

Montgomery et al J Biol Chem. 2012 Sep 28;287(40):33227-3

Insert

HMGCLL1 (HMGCLL1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
GFP-Braf R188L

Plasmid

#118330

Purpose

Expression of human Braf

Depositor

Philip Stork

Article

Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

Insert

Braf R188L (BRAF Human)

Tags

GFP

Expression

Mammalian

Mutation

R188L

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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nDHFR173-gs10-NLS-VPR

Plasmid

#188262

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nDHFR173 (DHFR Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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HA-HIF1a (S668A)

Plasmid

#49463

Purpose

HA-tagged HIF-1a Ser668 resuide mutated to Ala

Depositor

Wafik El-Deiry

Article

Warfel et al Cell Cycle. 2013 Oct 25;12(23).

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 668 to Alanine

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-Trib1

Plasmid

#131156

Purpose

Mammalian expression of flag-tagged mouse TRIB1

Depositor

Karen Keeshan, Warren Pear

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib1 (Trib1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pIKAT111

Plasmid

#69996

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

C1459T (R487C)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNAmyc-hPR-B-486-933

Plasmid

#89118

Purpose

mammalian expression of human progesterone receptor amino acid residues 486-933 with N-terminal myc tag, has PR DNA and ligand binding domains

Depositor

Elizabeth Wilson

Article

Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

Insert

human progesterone receptor ligand binding domain (PGR Human)

Tags

myc

Expression

Mammalian

Mutation

contains aa 486-933

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisA-HOXC6-1

Plasmid

#110366

Purpose

Mammalian expression of HisA-tagged HOXC6-1

Depositor

Carlos Moreno

Article

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Tags

HIS

Expression

Mammalian

Mutation

long isoform

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pIKAT127

Plasmid

#70005

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G444T (E148D)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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CITED2-T166N

Plasmid

#47749

Purpose

Introduces a T166N point mutation in the CITED2 gene

Depositor

Shoumo Bhattacharya

Article

Chen et al PLoS One. 2012;7(10):e46256. doi: 10.137

Insert

CITED2 (CITED2 Human)

Expression

Mammalian

Mutation

Threonine 166 to Asparagine

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pVeBtA2d7

Plasmid

#238989

Purpose

Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Hanson et al J Mol Biol. 2007 Apr 27;368(2):375-87. d

Insert

arrestin-2 long (ARRB2 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

deleted residues 174, 176, 177, 181-184

Promoter

CMV

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAK208_2xBRD9BD-IFP2.0C

Plasmid

#233734

Purpose

Expresses BRD9 Bromodomain as tandem double domain fused to C-terminal part of IFP2.0 for histone acetylation detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Genes (Basel). 2025 Apr 10;16(4):444. do

Insert

BRD9 (BRD9 Human)

Tags

IFP2.0 C-terminal part

Expression

Mammalian

Mutation

Bromodomain cloned twice in tandem

Promoter

CMV

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAK208_2xBRD9BD_Y57A-IFP2.0C

Plasmid

#233733

Purpose

Expresses BRD9 Bromodomain as tandem double domain Y57A mutant fused to C-terminal part of IFP2.0 as negative control for plasmid 233734

Depositor

Albert Jeltsch

Article

Kohler et al Genes (Basel). 2025 Apr 10;16(4):444. do

Insert

BRD9 (BRD9 Human)

Tags

IFP2.0 C-terminal part

Expression

Mammalian

Mutation

Bromodomain cloned twice in tandem with Y57A muta…

Promoter

CMV

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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p3VEA3-3A

Plasmid

#234797

Purpose

Mammalian expression of bovine arrestin-3 (I385A,V386A,F387A)

Depositor

Vsevolod Gurevich

Article

Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

I385A,V386A,F387A

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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