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Donor_C_FH_VIR

Plasmid

#186660

Purpose

Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Shu

Plasmid

#186655

Purpose

Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_Nbr

Plasmid

#186662

Purpose

Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_N_FH_GLE

Plasmid

#186657

Purpose

Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-mTert

Plasmid

#162555

Purpose

Overexpression of Tert

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of Tert (Tert Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

Plasmid

#58959

Purpose

lentiviral expression of v-H-Ras and luciferase/EGFP reporter

Depositor

Snorri Thorgeirsson

Article

Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

Inserts

CMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer/chicken beta actin

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR1A-HA-Jag1

Plasmid

#46052

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Entry vector

Tags

HA tag

Expression

Mutation

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-V5.mER.hOr47a

Plasmid

#126473

Purpose

Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Insert

Or47a (Or47a Fly)

Use

Tags

H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

Expression

Mammalian

Mutation

codon optimization for H. sapiens

Promoter

CMV

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

Plasmid

#128278

Purpose

Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

Depositor

Anna Sokac

Article

Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

Insert

twinstar (tsr) (tsr Fly)

Use

Tags

6X Histidine tag

Expression

Bacterial

Mutation

Met1 changed to Pro1; Cys77 silent basepair mutat…

Promoter

T7

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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