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CCR6-DuET
Plasmid#213202PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR6 (CCR6 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-Venus
Plasmid#139502PurposeExpresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PPO-mScarlet
Plasmid#139503PurposeExpresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.DepositorInsertparapinopsin
UseTagsmScarlet following Gly-Gly-Ser linkerExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gsi (Ser)
Plasmid#109352PurposeGsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGsi chimera
UseTagsNoneExpressionMammalianMutationChanged Cys at position 391 to Serine (Pertussis …PromoterCAGAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
UseTagsExpressionMammalianMutationPromoterPGK/U6Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWS931
Plasmid#123053PurposeTetR bacterial repressorDepositorInsertTetR-NLS [3a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-GINIP-Nluc
Plasmid#223556PurposePlasmid expressing GINIP-Nluc in mammalian cellsDepositorInserthuman GINIP - linker(GGGS) - Nluc
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
YFP-CAAX
Plasmid#223558PurposePlasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation boxDepositorInsertYFP(Venus) - KRas4b
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gst (Ser)
Plasmid#109354PurposeGsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGst chimera
UseTagsNoneExpressionMammalianMutationChanged Cysteine at position 391 to Serine (Pertu…PromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
