We narrowed to 4,189 results for: Pet2

1. pET28-H12TEV

   Plasmid

   #208635

   Purpose

   for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pet22b-mEGFP

   Plasmid

   #166439

   Purpose

   Bacterial expression of 6x His tagged mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21c(+)-mtLeuRS

   Plasmid

   #31079

   Depositor

   Linda Spremulli

   Article

   Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

   Insert

   Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pET22b_pScaC-SNAP_6xHis

   Plasmid

   #228835

   Purpose

   E. coli expression of the SNAP-tagged ScaC passenger domain

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   SNAPtag-TEV-6xHis

   Expression

   Bacterial

   Mutation

   aa 33-223

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SnoopCatcher

   Plasmid

   #72322

   Purpose

   Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a-SnoopCatcher
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed G842T and D848G of S. pneumoniae adhesin …

   Promoter

   T7

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b RNase A

   Plasmid

   #58903

   Purpose

   Bacterial expression of bovine pancreatic ribonuclease A

   Depositor

   Ronald Raines

   Article

   delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

   Insert

   RNase A (RNASE1 Bovine)
   

   Use

   Tags

   pelB sequence

   Expression

   Bacterial

   Mutation

   See Depositor Comments below

   Promoter

   T7

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a(+)::NL3F10H

   Plasmid

   #141290

   Purpose

   NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3) and His (x10)

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-pchG WT

   Plasmid

   #87107

   Purpose

   expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

   Insert

   pchG WT
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28b-irp3 WT

   Plasmid

   #87106

   Purpose

   expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

   Depositor

   Audrey Lamb

   Article

   Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

   Insert

   irp3 WT
   

   Use

   Tags

   his-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b-ELP[V]60

    Plasmid

    #219406

    Purpose

    (VPGVG)60

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    ELP[V]60
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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