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pAP118

Plasmid

#105495

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

HupA-SmBiT/HupA-LgBiT

Use

Atc-dependent expression in c. difficile

Tags

Expression

Mutation

Promoter

Ptet

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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BtGRK2-sYFP2

Plasmid

#137778

Purpose

Visualization of GRK2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Bovine GRK2 (GRK2 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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RnBarr2-mTurquoise2

Plasmid

#137792

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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RnBarr1-mTurquoise2

Plasmid

#137789

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro-{beta}4 SCR

Plasmid

#16267

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SCR Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
mVenus-RnBarr1

Plasmid

#137796

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-Flag-TRAF5

Plasmid

#102679

Purpose

retroviral expression plasmid for TRAF5

Depositor

Hasam Habelhah

Article

Zhang et al J Mol Biol. 2009 Jun 12;389(3):495-510.

Insert

TRAF5 (Traf5 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS EFF-1::V5

Plasmid

#133584

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

Insert

EFF-1::V5

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAT15416-BEAR-mScarlet-target-BFP

Plasmid

#162996

Purpose

plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-mScarlet plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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721-2µ-staHIS3

Plasmid

#40611

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1 C7G HA

Plasmid

#8954

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C7G: point mutation in LxCxE motif, LxghE

Promoter

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pFA-eCA

Plasmid

#133010

Purpose

pFA carrying a E. coli canA gene (Uniprot: P61517)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

Can (can E. coli)

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

tetR

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAT9753-BEAR-mScarlet-preedited

Plasmid

#162994

Purpose

BEAR control plasmid with split mScarlet and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT5R1AtR2NtB2

Plasmid

#160888

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T5

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-T5: T7, chaperones: T11

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT1R1AtR2NtB2

Plasmid

#160885

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T1

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-T1: T7, chaperones: T8

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS5R1AtR2NtB2

Plasmid

#160884

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-S5

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-S5: T7, chaperones: T7

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS2R1AtR2NtB2

Plasmid

#160883

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S2, rbcX, raf1, raf2, bsd2

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcS-S2: T7, chaperones: T7

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTH734-CEN-minHIS3

Plasmid

#38227

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Synthetic, Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTH737-CEN-maxHIS3

Plasmid

#38229

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Synthetic, Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3 (=GPD)

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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HOXD13 (chicken) HIS-tag pET

Plasmid

#8555

Depositor

Corey Largman

Article

Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

Insert

HOXD13 (HOXD13 )

Use

Tags

His and T7

Expression

Bacterial

Mutation

contains 10 nt 5' to ATG start; makes prote…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only