We narrowed to 621 results for: 1302

1. pJC45BS104 F130W

   Plasmid

   #1302

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 F130W (HSP104 Budding Yeast)
   

   Tags

   10X His tag

   Expression

   Bacterial

   Mutation

   Changed Phe 130 to Trp

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

   Plasmid

   #160627

   Purpose

   TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s-MS2:TV-Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

   Plasmid

   #160633

   Purpose

   TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31-RDF
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

   Plasmid

   #160622

   Purpose

   TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s_MS2:EDLL_Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST40-CES2A-ΔC-S227A

   Plasmid

   #200556

   Purpose

   Mammalian expression of secreted mouse CES2A S227A mutant

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
   

   Tags

   6xHis, Flag, and V5

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB3_Omega2_Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1678)

   Plasmid

   #160642

   Purpose

   Module for the dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   GRLacIBDGal4AD / RDF
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGB 35S:dCas9:Tnos (GB1191)

   Plasmid

   #68223

   Purpose

   Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   35S

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGB 35s:dCas:EDLL:tNos (GB1190)

   Plasmid

   #75404

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional Activator

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas:EDLL
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)

   Plasmid

   #160641

   Purpose

   Module for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   ERLexABDGal4AD / PhiC31
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGB3 alpha1 35s:PIF6:tNos (GB0170)

   Plasmid

   #160606

   Purpose

   TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

   Insert

   PIF6
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGB 35s:dCas:VP64:tNos (GB1189)

    Plasmid

    #75403

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas:VP64
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    35S

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-TDP-43 (4SA)

    Plasmid

    #235499

    Purpose

    Expression of human TDP-43-EGFP with phospho-dead mutations in the C-terminus

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S403A, S404A, S409A, S410A

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEGFP-TDP-43 (2KR)

    Plasmid

    #235494

    Purpose

    Expression of human TDP-43-EGFP with K136R and K408R mutations

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K136R, K408R

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pEGFP-TDP-43 (K408R)

    Plasmid

    #235493

    Purpose

    Expression of human TDP-43-EGFP with K408R mutation to block SUMOylation

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K408R

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

    Plasmid

    #160617

    Purpose

    TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    mini35s

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

    Plasmid

    #160609

    Purpose

    TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2-VP64:Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

    Plasmid

    #160614

    Purpose

    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    mini35s

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

    Plasmid

    #160615

    Purpose

    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    mini35s

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W

    Plasmid

    #105028

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W

    Plasmid

    #105029

    Purpose

    Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Slc25a51 (Slc25a51 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti HsATP13A3 WT

    Plasmid

    #195849

    Purpose

    Expresses wild-type Homo sapiens ATP13A3

    Depositor

    Veerle Baekelandt

    Article

    Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

    Insert

    ATPase 13A3 (ATP13A3 Human)
    

    Use

    Lentiviral

    Mutation

    D498N, L956P, M850I, V855M, R858H, L675V

    Promoter

    CMV

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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