We narrowed to 4,373 results for: 424

1. OoH_pET29b

   Plasmid

   #215424

   Purpose

   Expression construct for OoH gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   OoH
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dcp1 H206A-EGFP

   Plasmid

   #201424

   Purpose

   Bacterial protein expression of Dcp1 H206A

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dcp1 (DCP1 Budding Yeast)
   

   Tags

   EGFP, TEV, 6xHis and MBP, TEV

   Expression

   Bacterial

   Mutation

   H206A

   Promoter

   tac

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. JAB3605

   Plasmid

   #190424

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_2XPhlFO::GFPint-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_2XPhlFO::GFPint-ADHt
   

   Expression

   Plant

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. RVD-TT9

   Plasmid

   #173424

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Insert

   NG-NG dimer RVD at Position9
   

   Use

   Unspecified

   Mutation

   no

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP672_L2_pV01_0I_VloxP-OCS-Rluc_lacZ

   Plasmid

   #192424

   Purpose

   To act as the off state for testing of the alt VCre recombinase.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::VloxP-OCS-VloxP::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTL363

   Plasmid

   #168424

   Purpose

   pGBKT7 yeast two-hybrid plasmid

   Depositor

   Sophien Kamoun

   Article

   Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

   Insert

   OsaHIP10
   

   Expression

   Yeast

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKM328

   Plasmid

   #134243

   Purpose

   Mammalian expression construct expressing sgRNA against PLK1

   Depositor

   Iain Cheeseman

   Article

   Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

   Insert

   gRNA targeting PLK1 3'
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. S3_007_026_MV2

   Plasmid

   #134424

   Purpose

   pYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N_2xNLS
   

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. nucHCB1

   Plasmid

   #97424

   Purpose

   Nuclear Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP and nuclear localization signal.

   Depositor

   Ray Truant

   Article

   Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

   Insert

   nucHCB1
   

   Tags

   NLS and YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

    Plasmid

    #242407

    Purpose

    Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

    Depositor

    Ewan St. John Smith

    Article

    Lim et al bioRxiv 2024.09.24.614636

    Inserts

    mScarlet3
    IRES-EGFP
    IRES-moxBFP
    

    Use

    Self-amplifying rna

    Expression

    Mammalian

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti6-SLC7A11/xCT-V5

    Plasmid

    #170427

    Purpose

    Eepresses SLC7A11 (also named xCT) in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRIS

    Plasmid

    #120424

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3, IS150
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    constitutive

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE2-Bla(HA–RILP-FLAG)

    Plasmid

    #102424

    Purpose

    Dual tagged RILP for expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    Tet-Responsive

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE60 Q58L wgMDH

    Plasmid

    #204246

    Purpose

    Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Q58L

    Promoter

    T5

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid

    #84245

    Purpose

    Expresses direct fusion KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    KRAB-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, KRAB, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid

    #84247

    Purpose

    Expresses direct fusion VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, VPR, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-IRES-Gαi*-BERKY3

    Plasmid

    #158424

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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