Addgene

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pFA6a-link-yomTagBFP2-SpHis5

Plasmid

#44839

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

Plasmid

#180011

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

Plasmid

#180012

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-BFP-24xPP7

Plasmid

#74929

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-BFP-24xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

V5-mTagBFP2

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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#1.4 pre-R-SynapShot-TagBFP

Plasmid

#214719

Purpose

Expresses red #1.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 pre-R-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-3XBFPnls

Plasmid

#75384

Purpose

MCP-3XBFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

MCP

Use

Lentiviral

Tags

3XBFP

Expression

Mammalian

Mutation

Promoter

EFS

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 Peroxisome visual barcode

Plasmid

#158668

Purpose

tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomTagBFP-Kan

Plasmid

#44898

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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dCas9-10xSunTag-P2A-tagBFP

Plasmid

#174141

Purpose

Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

dCas9, 10xSunTag, tagBFP

Use

Tags

HA and tagBFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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TRE3G-PE2-P2A-BFP

Plasmid

#231580

Purpose

Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

Depositor

Jesse Engreitz

Article

Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

Insert

PE2-P2A-BFP

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL SFFV d20GFP.T2A.mTagBFP Donor

Plasmid

#31485

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d20BFP T2A BFP

Use

Lentiviral

Tags

Expression

Mutation

GFP has 20 amino acids deleted from the C terminu…

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-i53-bpA-EF1BFP

Plasmid

#111145

Purpose

Expression of i53 and a BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

i53 (mutL Synthetic)
BFP (BFP Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human Ubiquitin, contains 8 point mutations

Promoter

CAG promoter and EF1

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

Plasmid

#105043

Purpose

Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stk11 (Stk11 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

Plasmid

#159455

Purpose

Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

Plasmid

#67990

Purpose

CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab4a-c-Myc

Plasmid

#79799

Purpose

Express TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab4a (RAB4A Human)

Use

Tags

TagBFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMMLV-EBFP-P2A-Puro

Plasmid

#160227

Purpose

Expresses EBFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EBFP-P2A-Puro

Use

Retroviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

LTR

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail

Plasmid

#220079

Purpose

SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

SACM1l-C389S-Fis1tail (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

C389SdeltaTMD

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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