Addgene

We narrowed to 21,582 results for: Sis

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Article

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Rosa26-LSL-PE2 Targeting Vector

Plasmid

#196038

Purpose

The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Insert

Cas9-MMLV-RT

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET_His6-SUMO-TEV-TtCsm6

Plasmid

#172486

Purpose

Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)

Use

Tags

His-SUMO-TEV

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Use

Tags

His6 and bdSUMO

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-7xTRE-3xeGFP

Plasmid

#191206

Purpose

GFP expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 nonmutant

Plasmid

#183813

Purpose

Express CD74-ROS1 fusion (C6;R34) in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 G2032R

Plasmid

#183814

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Retroviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

G2032R

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 G2032R/L2086F

Plasmid

#183820

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R/L2086F mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

G2032R/L2086F

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 L2086F

Plasmid

#183816

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2086F mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

L2086F

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 S1986F/G2032R

Plasmid

#183821

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

S1986F/G2032R

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 L2000V

Plasmid

#183815

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

L2000V

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TAC-EB-C5

Plasmid

#80485

Purpose

piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 S1986F/L2000V

Plasmid

#183818

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2000V mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

S1986F/L2000V

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 S1986F/L2086F

Plasmid

#183819

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2086F mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

S1986F/L2086F

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-FRT-2xtTA

Plasmid

#191205

Purpose

Flpo dependent tTA expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

2xtTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 S1986F/G2032R/L2086F

Plasmid

#183822

Purpose

Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R/L2086F mutation in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Mutation

S1986F/G2032R/L2086F

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE59

Plasmid

#90404

Purpose

STAT3 - Sis-inducible element (SIE) gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

IRF1

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C-GFP

Plasmid

#195154

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C DCC-GFP

Plasmid

#195149

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove coi…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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